Engineering geminivirus resistance in Jatropha curcus

BACKGROUND: Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral disease outbreaks severely limit productivity. Therefore the development of...

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Bibliographic Details
Published in:Biotechnology for biofuels 2014, Vol.7 (1), p.149-149, Article 149
Main Authors: Ye, Jian, Qu, Jing, Mao, Hui-Zhu, Ma, Zhi-Gang, Rahman, Nur Estya Binte, Bai, Chao, Chen, Wen, Jiang, Shu-Ye, Ramachandran, Srinivasan, Chua, Nam-Hai
Format: Article
Language:English
Subjects:
Analysis
biodiesel
breeding
Cassava
Deoxyribonucleic acid
disease outbreaks
DNA
double-stranded RNA
Durability
engineering
Geminivirus
Gene expression
Genes
Genetic aspects
Genomes
Genomics
heritability
Indian cassava mosaic virus
Infiltration
Inoculation
Jatropha
Jatropha curcas
Laboratories
Life sciences
nucleotide sequences
nucleotides
Plants (organisms)
Polymerase chain reaction
progeny
Proteins
quantitative polymerase chain reaction
Ribonucleic acids
sequence homology
Strain
subtropics
Transgenic
transgenic plants
viruses
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Summary:BACKGROUND: Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral disease outbreaks severely limit productivity. Therefore the development of J. curcus to carry on durable virus resistance remains crucial and poses a major biotechnological challenge. RESULTS: We generated transgenic J. curcus plants expressing a hairpin, double-stranded (ds) RNA with sequences homologous to five key genes of ICMV-Dha strain DNA-A, which silences sequence-related viral genes thereby conferring ICMV resistance. Two rounds of virus inoculation were conducted via vacuum infiltration of ICMV-Dha. The durability and heritability of resistance conferred by the dsRNA was further tested to ascertain that T1 progeny transgenic plants were resistant to the ICMV-SG strain, which shared 94.5% nucleotides identity with the ICMV-Dha strain. Quantitative PCR analysis showed that resistant transgenic lines had no detectable virus. CONCLUSIONS: In this study we developed transgenic J. curcus plants to include a resistance to prevailing geminiviruses in Asia. These virus-resistant transgenic J. curcus plants can be used in various Jatropha breeding programs.
ISSN:1754-6834
1754-6834
DOI:10.1186/s13068-014-0149-z