Autophagy occurs within an hour of adenosine triphosphate treatment after nerve cell damage： the neuroprotective effects of adenosine triphosphate against apoptosis

• Published in: Neural regeneration research 2014-09, Vol.9 (17), p.1599-1605
• Main Authors: Lu, Na, Wang, Baoying, Deng, Xiaohui, Zhao, Honggang, Wang, Yong, Li, Dongliang
• Format: Article
• Language: English
• Subjects: Adenosine triphosphate; Apoptosis; Autophagy; Cell culture; Flow cytometry; Genetic aspects; Health aspects; Injuries; Nervous system; Neuroblastoma; Neurons; Physiology; Prevention; Proteins; SH-SY5Y细胞; Technical Updates; 三磷酸腺苷; 小时; 抗凋亡; 治疗; 神经保护作用; 细胞损伤; 自噬
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.141811

After hypoxia, ischemia, or inflammatory injuries to the central nervous system, the damaged cells release a large amount of adenosine triphosphate, which may cause secondary neuronal death. Autophagy is a form of cell death that also has neuroprotective effects. Cell Counting Kit assay, monodansylcadaverine staining, flow cytometry, western blotting, and real-time PCR were used to determine the effects of exogenous adenosine triphosphate treatment at different concentrations （2, 4, 6, 8, 10 mmol/L） over time （1, 2, 3, and 6 hours） on the apoptosis and autophagy of SH-SY5Y cells. High concentrations of extracellular adenosine triphosphate induced autophagy and apoptosis of SH-SYSY cells. The enhanced autophagy first appeared, and peaked at 1 hour after treatment with adenosine triphosphate. Cell apoptosis peaked at 3 hours, and persisted through 6 hours. With prolonged exposure to the adenosine triphosphate treatment, the fraction of apoptotic cells increased. These data suggest that the SH-SY5Y neural cells initiated autophagy against apoptosis within an hour of adenosine triphosphate treatment to protect themselves against injury.