The anthocyanin reduced Tomato Mutant Demonstrates the Role of Flavonols in Tomato Lateral Root and Root Hair Development

• Published in: Plant physiology (Bethesda) 2014-10, Vol.166 (2), p.614-631
• Main Authors: Maloney, Gregory S., DiNapoli, Kathleen T., Muday, Gloria K.
• Format: Article
• Language: English
• Subjects: anthocyanins; Auxins; Biological Transport; Biosynthesis; Enzymes; Flavonoids; Flavonols; Flavonols - biosynthesis; Flavonols - metabolism; Genes, Plant; Hypocotyls; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Lycopersicon esculentum - metabolism; mutants; Mutation; Plant roots; Plant Roots - growth & development; Plants; reactive oxygen species; Reactive Oxygen Species - metabolism; Root hairs; s - Focus Issue; Seedlings; tomatoes
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.114.240507

This study utilized tomato {Solarium lycopersicum) mutants with altered flavonoid biosynthesis to understand the impact of these metabolites on root development. The mutant anthocyanin reduced (are) has a mutation in the gene encoding FLAVONOID 3-HYDROXYLASE (F3H), the first step in flavonol synthesis, and accumulates higher concentrations of the F3H substrate, naringenin, and lower levels of the downstream products kaempferol, quercetin, myricetin, and anthocyanins, than the wild type. Complementation of are with the p35S:F3H transgene reduced naringenin and increased flavonols to wild-type levels. The initiation of lateral roots is reduced in are, and p35S:F3H complementation restores wild-type root formation. The flavonoid mutant anthocyanin without has a defect in the gene encoding DIHYDROFLAVONOL REDUCTASE, resulting in elevated flavonols and the absence of anthocyanins and displays increased lateral root formation. These results are consistent with a positive role of flavonols in lateral root formation. The are mutant has increased indole-S-acetic acid transport and greater sensitivity to the inhibitory effect of the auxin transport inhibitor naphthylphthalamic acid on lateral root formation. Expression of the auxin-induced reporter (DR5-β-glucuronidase) is reduced in initiating lateral roots and increased in primary root tips of are. Levels of reactive oxygen species are elevated in are root epidermal tissues and root hairs, and are forms more root hairs, consistent with a role of flavonols as antioxidants that modulate root hair formation. Together, these experiments identify positive roles of flavonols in the formation of lateral roots and negative roles in the formation of root hairs through the modulation of auxin transport and reactive oxygen species, respectively.