Oryeongsan inhibits LPS-induced production of inflammatory mediators via blockade of the NF-kappaB, MAPK pathways and leads to HO-1 induction in macrophage cells

• Published in: BMC complementary and alternative medicine 2014-07, Vol.14 (1), p.242-242, Article 242
• Main Authors: Oh, You-Chang, Jeong, Yun Hee, Ha, Jeong-Ho, Cho, Won-Kyung, Ma, Jin Yeul
• Format: Article
• Language: English
• Subjects: Aldosterone; Analysis; Animals; anti-inflammatory activity; Anti-Inflammatory Agents - pharmacology; Cell Line, Tumor; complement; Cytokines; Cytokines - metabolism; Development and progression; Enzyme Induction - drug effects; enzyme-linked immunosorbent assay; Enzymes; hematuria; Heme; heme oxygenase (biliverdin-producing); Heme Oxygenase-1 - biosynthesis; herbal medicines; inducible nitric oxide synthase; inflammation; Inflammation - drug therapy; Inflammation - metabolism; interleukin-1beta; Interleukins; Kidney diseases; Kinases; lipopolysaccharides; Lipopolysaccharides - antagonists & inhibitors; Lipopolysaccharides - pharmacology; macrophages; Macrophages - drug effects; Macrophages - enzymology; Macrophages - metabolism; MAP Kinase Signaling System - drug effects; Medical research; Medicine, Experimental; Membrane Proteins - biosynthesis; Mice; mitogen-activated protein kinase; Mitogen-Activated Protein Kinases - antagonists & inhibitors; Mitogen-Activated Protein Kinases - metabolism; Mitogens; nephritis; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; nitric oxide; Nitric Oxide Synthase Type II - metabolism; phosphorylation; Phosphorylation - drug effects; Physiological aspects; Plant Extracts - pharmacology; prostaglandin synthase; protective effect; Protein kinases; reverse transcriptase polymerase chain reaction; Rodents; Studies; therapeutics; traditional medicine; transcription factor NF-kappa B; tumor necrosis factors; Western blotting
• ISSN: 1472-6882; 1472-6882
• DOI: 10.1186/1472-6882-14-242

Oryeongsan (OR) is an herbal medication used in east-Asian traditional medicine to treat dysuresia, such as urinary frequency, hematuria, and dysuria due to renal disease and chronic nephritis. Recent studies showed that protective effect against acute gastric mucosal injury and an inhibitory effect on the renin-angiotensin-aldosterone pathway of OR. However, its effect on inflammation still remains unknown. In this study, to provide insight into the biological effects of OR, we investigated their effects on lipopolysaccharide (LPS)-mediated inflammation in the RAW 264.7 macrophage cells. We investigated the pharmacological and biological effects of OR on the production of pro-inflammatory cytokines, inflammatory mediators, and related products through Enzyme-linked immunosorbent assay (ELISA), reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Also, we examined the activation and suppression of nuclear factor (NF)-kappaB and mitogen-activated protein kinases (MAPKs) pathways in LPS-stimulated macrophages via Western blot analysis in order to explore inhibitory mechanism of OR. OR had anti-inflammatory effects by inhibiting the production of nitric oxide (NO), tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, and IL-1 beta. In addition, it strongly suppressed cyclooxygenase (COX)-2 and inducible nitric oxide synthase (iNOS), NO synthesizing enzymes. It also induced heme oxygenase (HO)-1 expression and inhibited NF-kappaB signaling pathway activation and phosphorylation of MAPKs. We further demonstrate the anti-inflammatory effects and inhibitory mechanism of OR in LPS-stimulated macrophages for the first time. OR contains strong anti-inflammatory activity and affects various mechanism pathways including NF-kappaB, MAPKs and HO-1. Our results suggest that OR has potential value to be developed as an inflammatory therapeutic agent from a natural substance.