Development of Asthmatic Response upon Bronchial Allergen Challenge Is Associated with Dynamic Changes of Interleukin-10-Producing and Interleukin-10-Responding CD4+ T Cells

• Published in: Inflammation 2014-12, Vol.37 (6), p.1945-1956
• Main Authors: Moniuszko, Marcin, Grubczak, Kamil, Kowal, Krzysztof, Eljaszewicz, Andrzej, Rusak, Malgorzata, Jeznach, Marta, Jablonska, Ewa, Dabrowska, Milena, Bodzenta-Lukaszyk, Anna
• Format: Article
• Language: English
• Subjects: Administration, Inhalation; Allergens - administration & dosage; Antigens, Dermatophagoides - administration & dosage; Asthma - blood; Asthma - chemically induced; Asthma - diagnosis; Biomarkers - blood; Biomedical and Life Sciences; Biomedicine; Bronchial Provocation Tests - methods; CD4-Positive T-Lymphocytes - metabolism; Cells, Cultured; Humans; Immunology; Interleukin-10 - blood; Internal Medicine; Pathology; Pharmacology/Toxicology; Rheumatology
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-014-9927-9

The role of adaptive immune system in regulation of asthmatic responses remains elusive. Here, we performed a comprehensive time-course analysis of mutual relationships between development of asthmatic response following allergen challenge and changes in several CD4+ T cell subsets which we characterized as either releasing interleukin-10 (CD4+CD25−CD127− and CD4+CD25+CD127+ T cells) or responding to IL-10 (CD4+ T cell subsets expressing CD210). Patients that developed asthmatic reaction were described as responders (R) whereas the others were named non-responders (NR). In R, in contrast to NR, at 6 h, we demonstrated significant expansion of CD4+CD25−CD127− T cells which was followed by drop to baseline values at 24 h. In contrast, in R, we observed decrease in numbers of CD4+CD25+CD127+ and CD4+CD25−CD127+ T cells at 24 h. Interestingly, at baseline, despite comparable IL-10 levels, R presented with lower levels of all CD4+ T cell subsets expressing CD210. In R, the numbers of CD4+CD210+ T cell subsets were further decreased following bronchial challenge which was paralleled by decrease in IL-10 serum levels. Altogether, our data suggest that dynamic interactions between IL-10-producing and IL-10-responding CD4+ T cells could contribute to pathogenesis of asthmatic responses in atopic individuals.