Regulatory T cell kinetics in the peripheral blood of patients with Crohn’s disease

Abstract Estimates of T regulatory cell populations in the periphery of patients with Crohn’s disease are confounded by disease activity and concomitant immunotherapeutic agents known to affect T cell proliferation and survival. We performed deuterium pulse/chase experiments in patients with quiesce...

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Published in:Human immunology 2013-02, Vol.74 (2), p.145-150
Main Authors: Rahman, Meher K, Offord, Chetan P, Khanna, Sahil, Ford, Godfrey C, Perrson, Xuan-Mai T, Svingen, Phyllis A, Xiong, Yuning, Bajzer, Zeljko, Faubion, William A
Format: Article
Language:English
Subjects:
Adult
Aged
Allergy and Immunology
Apoptosis - immunology
Blood Glucose
Case-Control Studies
Crohn Disease - immunology
Crohn Disease - metabolism
Female
Humans
Immunologic Memory
Lymphocyte Activation - immunology
Male
Middle Aged
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - metabolism
Young Adult
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Summary:Abstract Estimates of T regulatory cell populations in the periphery of patients with Crohn’s disease are confounded by disease activity and concomitant immunotherapeutic agents known to affect T cell proliferation and survival. We performed deuterium pulse/chase experiments in patients with quiescent Crohn’s disease on no immunotherapy and healthy control subjects to estimate T regulatory cell kinetics. Quantification of deuterated DNA isolated from T cell subsets over 10 days was determined by mass spectrophotometry. We demonstrate enhanced proliferation within the T regulatory cell population from patients with Crohn’s disease when compared to non-T regulatory cells and T regulatory cells from healthy control subjects. We speculate that T regulatory cells isolated from the periphery of patients with Crohn’s disease experience persistent antigen stimulation resulting in excess proliferative rates.
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2012.10.021