Effects of mouse utricle stromal tissues on hair cell induction from induced pluripotent stem cells

Hair cells are important for maintaining our sense of hearing and balance. However, they are difficult to regenerate in mammals once they are lost. Clarification of the molecular mechanisms underlying inner ear disorders is also impeded by the anatomical limitation of experimental access to the huma...

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Bibliographic Details
Published in:BMC neuroscience 2014-11, Vol.15 (1), p.121-121, Article 121
Main Authors: Taura, Akiko, Ohnishi, Hiroe, Ochi, Shohei, Ebisu, Fumi, Nakagawa, Takayuki, Ito, Juichi
Format: Article
Language:English
Subjects:
Analysis
Animals
Cell Culture Techniques
Cell Line
Coculture Techniques
Disease
Ears & hearing
Efficiency
Epithelium - physiology
Extracellular matrix
Extracellular Matrix - physiology
Hair
Hair Cells, Auditory - physiology
Immunohistochemistry
Induced Pluripotent Stem Cells - physiology
Medical research
Medicine, Experimental
Mice
Physiological aspects
Saccule and Utricle - cytology
Saccule and Utricle - physiology
Stem cells
Stromal Cells - physiology
Studies
Transplantation
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Summary:Hair cells are important for maintaining our sense of hearing and balance. However, they are difficult to regenerate in mammals once they are lost. Clarification of the molecular mechanisms underlying inner ear disorders is also impeded by the anatomical limitation of experimental access to the human inner ear. Therefore, the generation of hair cells, possibly from induced pluripotent stem (iPS) cells, is important for regenerative therapy and studies of inner ear diseases. We generated hair cells from mouse iPS cells using an established stepwise induction protocol. First, iPS cells were differentiated into the ectodermal lineage by floating culture. Next, they were treated with basic fibroblast growth factor to induce otic progenitor cells. Finally, the cells were co-cultured with three kinds of mouse utricle tissues: stromal tissue, stromal tissue + sensory epithelium, and the extracellular matrix of stromal tissue. Hair cell-like cells were successfully generated from iPS cells using mouse utricle stromal tissues. However, no hair cell-like cells with hair bundle-like structures were formed using other tissues. Hair cell-like cells were induced from mouse iPS cells using mouse utricle stromal tissues. Certain soluble factors from mouse utricle stromal cells might be important for induction of hair cells from iPS cells.
ISSN:1471-2202
1471-2202
DOI:10.1186/s12868-014-0121-7