Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma

• Published in: Journal of translational medicine 2014-07, Vol.12 (1), p.190-190, Article 190
• Main Authors: Wu, Jiao, Hao, Zhi-Wei, Zhao, You-Xu, Yang, Xiang-Min, Tang, Hao, Zhang, Xin, Song, Fei, Sun, Xiu-Xuan, Wang, Bin, Nan, Gang, Chen, Zhi-Nan, Bian, Huijie
• Format: Article
• Language: English
• Subjects: Adult; Aged; alpha-Fetoproteins - metabolism; Basigin - blood; Basigin - chemistry; Basigin - metabolism; Biology; Biomarkers, Tumor - blood; Biotechnology; Carcinoma, Hepatocellular - blood; Carcinoma, Hepatocellular - diagnosis; Carcinoma, Hepatocellular - enzymology; Carcinoma, Hepatocellular - pathology; Case-Control Studies; Cell Line, Tumor; Cell Membrane - metabolism; Culture Media, Conditioned - metabolism; Diagnosis; Enzyme-linked immunosorbent assay; Enzymes; Extracellular Signal-Regulated MAP Kinases - metabolism; Female; Focal Adhesion Protein-Tyrosine Kinases - metabolism; Genetic aspects; Hepatoma; Humans; Life sciences; Liver cancer; Liver Neoplasms - blood; Liver Neoplasms - diagnosis; Liver Neoplasms - enzymology; Liver Neoplasms - pathology; Male; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 2 - secretion; Metastasis; Middle Aged; Neoplasm Staging; Phosphatidylinositol 3-Kinases - metabolism; Physiological aspects; Protein Binding; Protein Structure, Tertiary; Proteins; Proto-Oncogene Proteins c-akt - metabolism; ROC Curve; Signal Transduction; Solubility; Up-Regulation
• ISSN: 1479-5876; 1479-5876
• DOI: 10.1186/1479-5876-12-190

As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnostic value of serum soluble CD147 in the HCC detection. We identified the form of soluble CD147 in cell culture supernate of HCC cells and serum of patients with HCC, and explored the role of soluble CD147 on MMPs secretion. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay, and the value of soluble CD147 as a marker in HCC detection was analyzed. Full length soluble CD147 was presented in the culture medium of HCC cells and serum of patients with HCC. The extracellular domain of soluble CD147 promoted the expression of CD147 and MMP-2 from HCC cells. Knockdown of CD147 markedly diminished the up-regulation of CD147 and MMP-2 which induced by soluble CD147. Soluble CD147 activated ERK, FAK, and PI3K/Akt pathways, leading to the up-regulation of MMP-2. The level of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy individuals (P