Loading…
Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma
As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnos...
Saved in:
| Published in: | Journal of translational medicine 2014-07, Vol.12 (1), p.190-190, Article 190 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873 |
|---|---|
| cites | cdi_FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873 |
| container_end_page | 190 |
| container_issue | 1 |
| container_start_page | 190 |
| container_title | Journal of translational medicine |
| container_volume | 12 |
| creator | Wu, Jiao Hao, Zhi-Wei Zhao, You-Xu Yang, Xiang-Min Tang, Hao Zhang, Xin Song, Fei Sun, Xiu-Xuan Wang, Bin Nan, Gang Chen, Zhi-Nan Bian, Huijie |
| description | As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnostic value of serum soluble CD147 in the HCC detection.
We identified the form of soluble CD147 in cell culture supernate of HCC cells and serum of patients with HCC, and explored the role of soluble CD147 on MMPs secretion. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay, and the value of soluble CD147 as a marker in HCC detection was analyzed.
Full length soluble CD147 was presented in the culture medium of HCC cells and serum of patients with HCC. The extracellular domain of soluble CD147 promoted the expression of CD147 and MMP-2 from HCC cells. Knockdown of CD147 markedly diminished the up-regulation of CD147 and MMP-2 which induced by soluble CD147. Soluble CD147 activated ERK, FAK, and PI3K/Akt pathways, leading to the up-regulation of MMP-2. The level of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy individuals (P |
| doi_str_mv | 10.1186/1479-5876-12-190 |
| format | article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4227008</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A539714227</galeid><sourcerecordid>A539714227</sourcerecordid><originalsourceid>FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873</originalsourceid><addsrcrecordid>eNp1Uk1v1DAQjRCIlsKdE7LEhUuK7fgrF6RqoYDUCg5wthxnsuvi2MFOEFz55ThsWVpU5INHM-89v5lxVT0l-JQQJV4SJtuaKylqQmvS4nvV8SF1_0Z8VD3K-QpjyjhrH1ZHlLWtEIwdVz_PF-9rD2E771COfuk8oM3rwkRTimOcIaPLy481RfB9SpCziwGZ0COXkUFTqYfZGY8ypOjj1tkSjyZ9gYRcQD3MYOeVEge0g8nM0YL3izcJWZOsC3E0j6sHg_EZnlzfJ9Xn8zefNu_qiw9v32_OLupOEDnXljOKuaB8kD1TMAwWt7wTvG_6wQCnvWk4VaSVXVNi0bLegMJCQEeVEko2J9Wrve60dCP0tjhPxuspuWL4h47G6duV4HZ6G79pRqnEWBWBzV6gc_E_ArcrNo563YFed6AJ1WVFReXFtY0Uvy6QZz26vE7FBIhL1oTzRuJGUl6gz_-BXsUlhTKk3yjGqJL4L2prPGgXhlget6uoPuNNK8nqv6BO70CV08PobAwwuJK_RcB7gk0x5wTDoVGC9fr77mrt2c0JHwh_vlvzCxtQ1Zs</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1553442870</pqid></control><display><type>article</type><title>Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Wu, Jiao ; Hao, Zhi-Wei ; Zhao, You-Xu ; Yang, Xiang-Min ; Tang, Hao ; Zhang, Xin ; Song, Fei ; Sun, Xiu-Xuan ; Wang, Bin ; Nan, Gang ; Chen, Zhi-Nan ; Bian, Huijie</creator><creatorcontrib>Wu, Jiao ; Hao, Zhi-Wei ; Zhao, You-Xu ; Yang, Xiang-Min ; Tang, Hao ; Zhang, Xin ; Song, Fei ; Sun, Xiu-Xuan ; Wang, Bin ; Nan, Gang ; Chen, Zhi-Nan ; Bian, Huijie</creatorcontrib><description>As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnostic value of serum soluble CD147 in the HCC detection.
We identified the form of soluble CD147 in cell culture supernate of HCC cells and serum of patients with HCC, and explored the role of soluble CD147 on MMPs secretion. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay, and the value of soluble CD147 as a marker in HCC detection was analyzed.
Full length soluble CD147 was presented in the culture medium of HCC cells and serum of patients with HCC. The extracellular domain of soluble CD147 promoted the expression of CD147 and MMP-2 from HCC cells. Knockdown of CD147 markedly diminished the up-regulation of CD147 and MMP-2 which induced by soluble CD147. Soluble CD147 activated ERK, FAK, and PI3K/Akt pathways, leading to the up-regulation of MMP-2. The level of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy individuals (P < 0.001). Soluble CD147 levels were found to be associated with HCC tumor size (P = 0.007) and Child-Pugh grade (P = 0.007). Moreover, soluble CD147 showed a better performance in distinguishing HCC compared with alpha-fetoprotein.
The extracellular domain of soluble CD147 enhances the secretion of MMP-2 from HCC cells, requiring the cooperation of membrane CD147 and activation of ERK, FAK, and PI3K/Akt signaling. The measurement of soluble CD147 may offer a useful approach in diagnosis of HCC.</description><identifier>ISSN: 1479-5876</identifier><identifier>EISSN: 1479-5876</identifier><identifier>DOI: 10.1186/1479-5876-12-190</identifier><identifier>PMID: 24996644</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Aged ; alpha-Fetoproteins - metabolism ; Basigin - blood ; Basigin - chemistry ; Basigin - metabolism ; Biology ; Biomarkers, Tumor - blood ; Biotechnology ; Carcinoma, Hepatocellular - blood ; Carcinoma, Hepatocellular - diagnosis ; Carcinoma, Hepatocellular - enzymology ; Carcinoma, Hepatocellular - pathology ; Case-Control Studies ; Cell Line, Tumor ; Cell Membrane - metabolism ; Culture Media, Conditioned - metabolism ; Diagnosis ; Enzyme-linked immunosorbent assay ; Enzymes ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Female ; Focal Adhesion Protein-Tyrosine Kinases - metabolism ; Genetic aspects ; Hepatoma ; Humans ; Life sciences ; Liver cancer ; Liver Neoplasms - blood ; Liver Neoplasms - diagnosis ; Liver Neoplasms - enzymology ; Liver Neoplasms - pathology ; Male ; Matrix Metalloproteinase 2 - metabolism ; Matrix Metalloproteinase 2 - secretion ; Metastasis ; Middle Aged ; Neoplasm Staging ; Phosphatidylinositol 3-Kinases - metabolism ; Physiological aspects ; Protein Binding ; Protein Structure, Tertiary ; Proteins ; Proto-Oncogene Proteins c-akt - metabolism ; ROC Curve ; Signal Transduction ; Solubility ; Up-Regulation</subject><ispartof>Journal of translational medicine, 2014-07, Vol.12 (1), p.190-190, Article 190</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Wu et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Copyright © 2014 Wu et al.; licensee BioMed Central Ltd. 2014 Wu et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873</citedby><cites>FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227008/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1553442870?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24996644$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Jiao</creatorcontrib><creatorcontrib>Hao, Zhi-Wei</creatorcontrib><creatorcontrib>Zhao, You-Xu</creatorcontrib><creatorcontrib>Yang, Xiang-Min</creatorcontrib><creatorcontrib>Tang, Hao</creatorcontrib><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Song, Fei</creatorcontrib><creatorcontrib>Sun, Xiu-Xuan</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Nan, Gang</creatorcontrib><creatorcontrib>Chen, Zhi-Nan</creatorcontrib><creatorcontrib>Bian, Huijie</creatorcontrib><title>Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma</title><title>Journal of translational medicine</title><addtitle>J Transl Med</addtitle><description>As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnostic value of serum soluble CD147 in the HCC detection.
We identified the form of soluble CD147 in cell culture supernate of HCC cells and serum of patients with HCC, and explored the role of soluble CD147 on MMPs secretion. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay, and the value of soluble CD147 as a marker in HCC detection was analyzed.
Full length soluble CD147 was presented in the culture medium of HCC cells and serum of patients with HCC. The extracellular domain of soluble CD147 promoted the expression of CD147 and MMP-2 from HCC cells. Knockdown of CD147 markedly diminished the up-regulation of CD147 and MMP-2 which induced by soluble CD147. Soluble CD147 activated ERK, FAK, and PI3K/Akt pathways, leading to the up-regulation of MMP-2. The level of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy individuals (P < 0.001). Soluble CD147 levels were found to be associated with HCC tumor size (P = 0.007) and Child-Pugh grade (P = 0.007). Moreover, soluble CD147 showed a better performance in distinguishing HCC compared with alpha-fetoprotein.
The extracellular domain of soluble CD147 enhances the secretion of MMP-2 from HCC cells, requiring the cooperation of membrane CD147 and activation of ERK, FAK, and PI3K/Akt signaling. The measurement of soluble CD147 may offer a useful approach in diagnosis of HCC.</description><subject>Adult</subject><subject>Aged</subject><subject>alpha-Fetoproteins - metabolism</subject><subject>Basigin - blood</subject><subject>Basigin - chemistry</subject><subject>Basigin - metabolism</subject><subject>Biology</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biotechnology</subject><subject>Carcinoma, Hepatocellular - blood</subject><subject>Carcinoma, Hepatocellular - diagnosis</subject><subject>Carcinoma, Hepatocellular - enzymology</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Case-Control Studies</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - metabolism</subject><subject>Culture Media, Conditioned - metabolism</subject><subject>Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Female</subject><subject>Focal Adhesion Protein-Tyrosine Kinases - metabolism</subject><subject>Genetic aspects</subject><subject>Hepatoma</subject><subject>Humans</subject><subject>Life sciences</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - blood</subject><subject>Liver Neoplasms - diagnosis</subject><subject>Liver Neoplasms - enzymology</subject><subject>Liver Neoplasms - pathology</subject><subject>Male</subject><subject>Matrix Metalloproteinase 2 - metabolism</subject><subject>Matrix Metalloproteinase 2 - secretion</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Phosphatidylinositol 3-Kinases - metabolism</subject><subject>Physiological aspects</subject><subject>Protein Binding</subject><subject>Protein Structure, Tertiary</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>ROC Curve</subject><subject>Signal Transduction</subject><subject>Solubility</subject><subject>Up-Regulation</subject><issn>1479-5876</issn><issn>1479-5876</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp1Uk1v1DAQjRCIlsKdE7LEhUuK7fgrF6RqoYDUCg5wthxnsuvi2MFOEFz55ThsWVpU5INHM-89v5lxVT0l-JQQJV4SJtuaKylqQmvS4nvV8SF1_0Z8VD3K-QpjyjhrH1ZHlLWtEIwdVz_PF-9rD2E771COfuk8oM3rwkRTimOcIaPLy481RfB9SpCziwGZ0COXkUFTqYfZGY8ypOjj1tkSjyZ9gYRcQD3MYOeVEge0g8nM0YL3izcJWZOsC3E0j6sHg_EZnlzfJ9Xn8zefNu_qiw9v32_OLupOEDnXljOKuaB8kD1TMAwWt7wTvG_6wQCnvWk4VaSVXVNi0bLegMJCQEeVEko2J9Wrve60dCP0tjhPxuspuWL4h47G6duV4HZ6G79pRqnEWBWBzV6gc_E_ArcrNo563YFed6AJ1WVFReXFtY0Uvy6QZz26vE7FBIhL1oTzRuJGUl6gz_-BXsUlhTKk3yjGqJL4L2prPGgXhlget6uoPuNNK8nqv6BO70CV08PobAwwuJK_RcB7gk0x5wTDoVGC9fr77mrt2c0JHwh_vlvzCxtQ1Zs</recordid><startdate>20140704</startdate><enddate>20140704</enddate><creator>Wu, Jiao</creator><creator>Hao, Zhi-Wei</creator><creator>Zhao, You-Xu</creator><creator>Yang, Xiang-Min</creator><creator>Tang, Hao</creator><creator>Zhang, Xin</creator><creator>Song, Fei</creator><creator>Sun, Xiu-Xuan</creator><creator>Wang, Bin</creator><creator>Nan, Gang</creator><creator>Chen, Zhi-Nan</creator><creator>Bian, Huijie</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140704</creationdate><title>Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma</title><author>Wu, Jiao ; Hao, Zhi-Wei ; Zhao, You-Xu ; Yang, Xiang-Min ; Tang, Hao ; Zhang, Xin ; Song, Fei ; Sun, Xiu-Xuan ; Wang, Bin ; Nan, Gang ; Chen, Zhi-Nan ; Bian, Huijie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>alpha-Fetoproteins - metabolism</topic><topic>Basigin - blood</topic><topic>Basigin - chemistry</topic><topic>Basigin - metabolism</topic><topic>Biology</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biotechnology</topic><topic>Carcinoma, Hepatocellular - blood</topic><topic>Carcinoma, Hepatocellular - diagnosis</topic><topic>Carcinoma, Hepatocellular - enzymology</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Case-Control Studies</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - metabolism</topic><topic>Culture Media, Conditioned - metabolism</topic><topic>Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Female</topic><topic>Focal Adhesion Protein-Tyrosine Kinases - metabolism</topic><topic>Genetic aspects</topic><topic>Hepatoma</topic><topic>Humans</topic><topic>Life sciences</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - blood</topic><topic>Liver Neoplasms - diagnosis</topic><topic>Liver Neoplasms - enzymology</topic><topic>Liver Neoplasms - pathology</topic><topic>Male</topic><topic>Matrix Metalloproteinase 2 - metabolism</topic><topic>Matrix Metalloproteinase 2 - secretion</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Phosphatidylinositol 3-Kinases - metabolism</topic><topic>Physiological aspects</topic><topic>Protein Binding</topic><topic>Protein Structure, Tertiary</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>ROC Curve</topic><topic>Signal Transduction</topic><topic>Solubility</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Jiao</creatorcontrib><creatorcontrib>Hao, Zhi-Wei</creatorcontrib><creatorcontrib>Zhao, You-Xu</creatorcontrib><creatorcontrib>Yang, Xiang-Min</creatorcontrib><creatorcontrib>Tang, Hao</creatorcontrib><creatorcontrib>Zhang, Xin</creatorcontrib><creatorcontrib>Song, Fei</creatorcontrib><creatorcontrib>Sun, Xiu-Xuan</creatorcontrib><creatorcontrib>Wang, Bin</creatorcontrib><creatorcontrib>Nan, Gang</creatorcontrib><creatorcontrib>Chen, Zhi-Nan</creatorcontrib><creatorcontrib>Bian, Huijie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Jiao</au><au>Hao, Zhi-Wei</au><au>Zhao, You-Xu</au><au>Yang, Xiang-Min</au><au>Tang, Hao</au><au>Zhang, Xin</au><au>Song, Fei</au><au>Sun, Xiu-Xuan</au><au>Wang, Bin</au><au>Nan, Gang</au><au>Chen, Zhi-Nan</au><au>Bian, Huijie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma</atitle><jtitle>Journal of translational medicine</jtitle><addtitle>J Transl Med</addtitle><date>2014-07-04</date><risdate>2014</risdate><volume>12</volume><issue>1</issue><spage>190</spage><epage>190</epage><pages>190-190</pages><artnum>190</artnum><issn>1479-5876</issn><eissn>1479-5876</eissn><abstract>As a surface glycoprotein, CD147 is capable of stimulating the production of matrix metalloproteinases (MMPs) from neighboring fibroblasts. The aim of the present study is to explore the role of soluble CD147 on MMPs secretion from hepatocellular carcinoma (HCC) cells, and to investigate the diagnostic value of serum soluble CD147 in the HCC detection.
We identified the form of soluble CD147 in cell culture supernate of HCC cells and serum of patients with HCC, and explored the role of soluble CD147 on MMPs secretion. Serum CD147 levels were detected by the enzyme-linked immunosorbent assay, and the value of soluble CD147 as a marker in HCC detection was analyzed.
Full length soluble CD147 was presented in the culture medium of HCC cells and serum of patients with HCC. The extracellular domain of soluble CD147 promoted the expression of CD147 and MMP-2 from HCC cells. Knockdown of CD147 markedly diminished the up-regulation of CD147 and MMP-2 which induced by soluble CD147. Soluble CD147 activated ERK, FAK, and PI3K/Akt pathways, leading to the up-regulation of MMP-2. The level of soluble CD147 in serum of patients with HCC was significantly elevated compared with healthy individuals (P < 0.001). Soluble CD147 levels were found to be associated with HCC tumor size (P = 0.007) and Child-Pugh grade (P = 0.007). Moreover, soluble CD147 showed a better performance in distinguishing HCC compared with alpha-fetoprotein.
The extracellular domain of soluble CD147 enhances the secretion of MMP-2 from HCC cells, requiring the cooperation of membrane CD147 and activation of ERK, FAK, and PI3K/Akt signaling. The measurement of soluble CD147 may offer a useful approach in diagnosis of HCC.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>24996644</pmid><doi>10.1186/1479-5876-12-190</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1479-5876 |
| ispartof | Journal of translational medicine, 2014-07, Vol.12 (1), p.190-190, Article 190 |
| issn | 1479-5876 1479-5876 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4227008 |
| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Adult Aged alpha-Fetoproteins - metabolism Basigin - blood Basigin - chemistry Basigin - metabolism Biology Biomarkers, Tumor - blood Biotechnology Carcinoma, Hepatocellular - blood Carcinoma, Hepatocellular - diagnosis Carcinoma, Hepatocellular - enzymology Carcinoma, Hepatocellular - pathology Case-Control Studies Cell Line, Tumor Cell Membrane - metabolism Culture Media, Conditioned - metabolism Diagnosis Enzyme-linked immunosorbent assay Enzymes Extracellular Signal-Regulated MAP Kinases - metabolism Female Focal Adhesion Protein-Tyrosine Kinases - metabolism Genetic aspects Hepatoma Humans Life sciences Liver cancer Liver Neoplasms - blood Liver Neoplasms - diagnosis Liver Neoplasms - enzymology Liver Neoplasms - pathology Male Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 2 - secretion Metastasis Middle Aged Neoplasm Staging Phosphatidylinositol 3-Kinases - metabolism Physiological aspects Protein Binding Protein Structure, Tertiary Proteins Proto-Oncogene Proteins c-akt - metabolism ROC Curve Signal Transduction Solubility Up-Regulation |
| title | Full-length soluble CD147 promotes MMP-2 expression and is a potential serological marker in detection of hepatocellular carcinoma |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T09%3A29%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Full-length%20soluble%20CD147%20promotes%20MMP-2%20expression%20and%20is%20a%20potential%20serological%20marker%20in%20detection%20of%20hepatocellular%20carcinoma&rft.jtitle=Journal%20of%20translational%20medicine&rft.au=Wu,%20Jiao&rft.date=2014-07-04&rft.volume=12&rft.issue=1&rft.spage=190&rft.epage=190&rft.pages=190-190&rft.artnum=190&rft.issn=1479-5876&rft.eissn=1479-5876&rft_id=info:doi/10.1186/1479-5876-12-190&rft_dat=%3Cgale_pubme%3EA539714227%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b617t-c54205625f7d48effc095b65d3dfae52da3528197b32da694dae8066eb2886873%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1553442870&rft_id=info:pmid/24996644&rft_galeid=A539714227&rfr_iscdi=true |