Enhanced Chondrogenic Differentiation of Dental Pulp Stem Cells Using Nanopatterned PEG-GelMA-HA Hydrogels

We have examined the effects of surface nanotopography and hyaluronic acid (HA) on in vitro chondrogenesis of dental pulp stem cells (DPSCs). Ultraviolet-assisted capillary force lithography was employed to fabricate well-defined nanostructured scaffolds of composite PEG-GelMA-HA hydrogels that cons...

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Bibliographic Details
Published in:Tissue engineering. Part A 2014-11, Vol.20 (21-22), p.2817-2829
Main Authors: Nemeth, Cameron L., Janebodin, Kajohnkiart, Yuan, Alex E., Dennis, James E., Reyes, Morayma, Kim, Deok-Ho
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Biocompatible Materials - chemical synthesis
Biomedical materials
Cell Differentiation - physiology
Cell Proliferation - physiology
Cell Size
Cell Survival - physiology
Cells, Cultured
Chondrogenesis - physiology
Dental Pulp - cytology
Dental Pulp - physiology
Gene expression
Hyaluronic Acid - chemistry
Hydrogels
Hydrogels - chemistry
Materials Testing
Methacrylates - chemistry
Mice
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology
Original
Original Articles
Polyethylene Glycols - chemistry
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Surface Properties
Tissue engineering
Tissue Engineering - methods
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Summary:We have examined the effects of surface nanotopography and hyaluronic acid (HA) on in vitro chondrogenesis of dental pulp stem cells (DPSCs). Ultraviolet-assisted capillary force lithography was employed to fabricate well-defined nanostructured scaffolds of composite PEG-GelMA-HA hydrogels that consist of poly(ethylene glycol) dimethacrylate (PEGDMA), methacrylated gelatin (GelMA), and HA. Using this microengineered platform, we first demonstrated that DPSCs formed three-dimensional spheroids, which provide an appropriate environment for in vitro chondrogenic differentiation. We also found that DPSCs cultured on nanopatterned PEG-GelMA-HA scaffolds showed a significant upregulation of the chondrogenic gene markers ( Sox9, Alkaline phosphatase, Aggrecan, Procollagen type II, and Procollagen type X ), while downregulating the pluripotent stem cell gene, Nanog, and epithelial–mesenchymal genes ( Twist, Snail, Slug ) compared with tissue culture polystyrene-cultured DPSCs. Immunocytochemistry showed more extensive deposition of collagen type II in DPSCs cultured on the nanopatterned PEG-GelMA-HA scaffolds. These findings suggest that nanotopography and HA provide important cues for promoting chondrogenic differentiation of DPSCs.
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2013.0614