Three human chromosomal autoantigens are recognized by sera from patients with anti-centromere antibodies

We have identified 39 individuals with anti-centromere antibodies (ACA) in our patient population, all of whom have Raynaud's syndrome or disease. We have used sera from the ACA-positive patients and from 123 controls (22 normal individuals and 101 additional patients with either Raynaud's...

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Bibliographic Details
Published in:The Journal of clinical investigation 1986-02, Vol.77 (2), p.426-430
Main Authors: Earnshaw, W, Bordwell, B, Marino, C, Rothfield, N
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Antinuclear - analysis
Antigens - immunology
Autoantibodies - analysis
Autoantibodies - immunology
Autoantigens - immunology
Centromere - immunology
Chromosomes - immunology
Connective Tissue Diseases - complications
Connective Tissue Diseases - immunology
Electrophoresis, Polyacrylamide Gel
Female
Histones - immunology
Humans
Immunosorbent Techniques
Male
Middle Aged
Raynaud Disease - complications
Raynaud Disease - immunology
Scleroderma, Systemic - immunology
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Summary:We have identified 39 individuals with anti-centromere antibodies (ACA) in our patient population, all of whom have Raynaud's syndrome or disease. We have used sera from the ACA-positive patients and from 123 controls (22 normal individuals and 101 additional patients with either Raynaud's disease or Raynaud's syndrome plus an associated connective tissue disease) to screen the proteins of highly purified human (HeLa) mitotic chromosomes by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting. Three antigens were recognized by the sera from the ACA-positive patients. These were centromere protein (CENP)-B (80,000 mol wt--recognized by all ACA-positive sera), CENP-A (17,000 mol wt--recognized by 38 of 39 ACA-positive sera), and CENP-C (140,000 mol wt--recognized by 37 of 39 ACA-positive sera). None of these antigens were recognized by any of the 123 control sera, although binding was occasionally seen to other chromosomal antigens. Therefore the ACA response is highly uniform in our patient population. Antibody to CENP-B shows a 100% correlation with anti-centromere staining by indirect immunofluorescence.
ISSN:0021-9738
DOI:10.1172/JCI112320