Evaluation of serum lysyl oxidase as a blood test for colorectal cancer

Abstract Aims Lysyl oxidase (LOX) expression is elevated in colorectal cancer (CRC) tissue and associated with disease progression. A blood test may form a more acceptable diagnostic test for CRC although LOX has not previously been measured in the serum. We therefore sought to determine the clinica...

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Bibliographic Details
Published in:European journal of surgical oncology 2014-06, Vol.40 (6), p.731-738
Main Authors: Ward, S.T, Weston, C.J, Hepburn, E, Damery, S, Hejmadi, R.K, Morton, D.G, Middleton, G, Ismail, T, Adams, D.H
Format: Article
Language:English
Subjects:
Adult
Aged
Colorectal cancer
Colorectal Neoplasms - blood
Diagnosis
Disease Progression
Enzyme-Linked Immunosorbent Assay
Female
Hematology, Oncology and Palliative Medicine
Humans
Immunohistochemistry
Lysyl oxidase
Male
Middle Aged
Protein-Lysine 6-Oxidase - blood
Serum
Surgery
Surveys and Questionnaires
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Summary:Abstract Aims Lysyl oxidase (LOX) expression is elevated in colorectal cancer (CRC) tissue and associated with disease progression. A blood test may form a more acceptable diagnostic test for CRC although LOX has not previously been measured in the serum. We therefore sought to determine the clinical usefulness of a serum LOX test for CRC in a symptomatic population. Methods Adult patients referred to a hospital colorectal clinic with bowel symptoms completed a questionnaire and provided a blood sample for serum LOX measurement. Associations between presenting symptoms, serum LOX concentrations and outcomes of investigations were tested by univariate and multivariate analyses to determine if serum LOX was clinically useful in the prediction of CRC. LOX expression in CRC and adjacent colon biopsies was evaluated by ELISA and immunohistochemistry. Results Thirty-one cases of colorectal cancer and 16 high-risk polyps were identified from a total of 962 participants. There was no association between serum LOX concentration and the presence of CRC, high-risk polyps or cancers at any site. LOX expression was significantly increased in CRC tissue compared to adjacent colon. Conclusion Despite overexpression of LOX in CRC tissue, elevated serum levels could not be demonstrated. Serum LOX measurement is therefore not a clinically useful test for CRC.
ISSN:0748-7983
1532-2157
DOI:10.1016/j.ejso.2013.10.023