Role of shear rate and platelets in promoting fibrin formation on rabbit subendothelium: studies utilizing patients with quantitative and qualitative platelet defects

The deposition of platelets on subendothelium of rabbit aortic segments exposed to non-anticoagulated human blood increased progressively with increasing wall shear rates (50-2,600 s-1), whereas fibrin deposition decreased. Studies in normal subjects and patients with platelet disorders suggested th...

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Bibliographic Details
Published in:The Journal of clinical investigation 1986-10, Vol.78 (4), p.1072-1082
Main Authors: WEISS, H. J, TURITTO, V. T, BAUMGARTNER, H. R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blood coagulation. Blood cells
Blood Platelet Disorders - blood
Blood Platelets - physiology
Endothelium - ultrastructure
Female
Fibrin - biosynthesis
Fundamental and applied biological sciences. Psychology
Humans
In Vitro Techniques
Middle Aged
Molecular and cellular biology
Platelet
Platelet Count
Platelet Membrane Glycoproteins - deficiency
Thrombasthenia - blood
Time Factors
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Summary:The deposition of platelets on subendothelium of rabbit aortic segments exposed to non-anticoagulated human blood increased progressively with increasing wall shear rates (50-2,600 s-1), whereas fibrin deposition decreased. Studies in normal subjects and patients with platelet disorders suggested that, under the conditions used, platelets were essential for fibrin deposition at intermediate (650 s-1) but not low (50 s-1) shear rates. Fibrin deposition was markedly diminished in a patient with Scott syndrome whose platelets have a diminished capacity to bind Factor Xa and activate Factors IX and II. In glycoprotein IIb-IIIa deficiency, fibrin deposition was normal (or somewhat increased), whereas in glycoprotein Ib deficiency the association of fibrin with platelets, but not subendothelium, was decreased. The findings indicate that platelets, perhaps through surface localization of coagulation proteins, promote fibrin deposition on subendothelium at arterial shear rates and suggest that agents directed against platelet coagulant properties could be antithrombotic.
ISSN:0021-9738
1558-8238
DOI:10.1172/JCI112663