MiR-133b regulates bladder cancer cell proliferation and apoptosis by targeting Bcl-w and Akt1

MiR-133b is a muscle-specific microRNA; it has a role in the formation of cardiocytes and the expression of myocardium ion channels by regulating target genes. Many human malignant tumors demonstrate a low expression of miR-133b, as noted in colorectal, lung, esophagus and bladder cancers, but the r...

Full description

Saved in:
Bibliographic Details
Published in:Cancer cell international 2014-07, Vol.14 (1), p.70-70, Article 70
Main Authors: Chen, Xiao-Nan, Wang, Ke-Feng, Xu, Zhen-Qun, Li, Shi-Jie, Liu, Qiang, Fu, Dong-Hui, Wang, Xia, Wu, Bin
Format: Article
Language:English
Subjects:
Apoptosis
Bladder cancer
Cell growth
Flow cytometry
Gene expression
Kinases
Medical research
MicroRNAs
Molecular weight
Primary Research
Proteins
Rodents
Software
Statistical analysis
Studies
Tumors
Variance analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MiR-133b is a muscle-specific microRNA; it has a role in the formation of cardiocytes and the expression of myocardium ion channels by regulating target genes. Many human malignant tumors demonstrate a low expression of miR-133b, as noted in colorectal, lung, esophagus and bladder cancers, but the role of miR-133b in bladder cancer is unknown. The expression of miR-133b in clinical bladder cancer specimens and adjacent normal tissues was confirmed by stem-loop RT-PCR. We also analyzed the relationship between miR-133b expression and clinicopathological factors of bladder cancer. Bcl-w and Akt1 protein expression in 41 bladder cancer specimens and adjacent normal tissues was detected by Western blot. After transfection of miR-133b mimics or inhibitor into a T24 human bladder cancer cell line, Bcl-w and Akt1 protein and mRNA expression were examined by Western blot and RT-PCR, respectively. The effect of miR-133b on T24 cell proliferation and apoptosis was measured by CCK-8 tests and flow cytometry, respectively. The expression of miR-133b in bladder cancer tissues from 41 patients was significantly down-regulated (P 
ISSN:1475-2867
1475-2867
DOI:10.1186/s12935-014-0070-3