Immunological and Biological Evidence for a Stanniocalcin-Like Hormone in Human Kidney

The corpuscles of Stannius are responsible for the synthesis and secretion of stanniocalcin (STC), a glycoprotein hormone that regulates calcium and phosphate homeostasis in fishes through its actions on the gills and kidneys. The corpuscles of Stannius and STC are considered to be an endocrine syst...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1995-03, Vol.92 (6), p.1871-1875
Main Authors: Wagner, Graham F., Guiraudon, Colette C., Milliken, Christine, Copp, D. Harold
Format: Article
Language:English
Subjects:
Anatomy & physiology
Animals
Antiserum
Biological Assay
Biopsy
Blotting, Western
Calcium - metabolism
Female
Fish
Fishes
Gills
Gills - drug effects
Gills - metabolism
Glycoproteins
Glycoproteins - analysis
Glycoproteins - isolation & purification
Glycoproteins - pharmacology
Hagfishes
Hormones
Hormones - analysis
Hormones - isolation & purification
Hormones - pharmacology
Humans
Immunohistochemistry
Kidney - chemistry
Kidney - cytology
Kidney - pathology
Kidneys
Male
Rats
Rats, Sprague-Dawley
Salamanders
Salmon
Sharks
Trout
Urodela
Vertebrates
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Summary:The corpuscles of Stannius are responsible for the synthesis and secretion of stanniocalcin (STC), a glycoprotein hormone that regulates calcium and phosphate homeostasis in fishes through its actions on the gills and kidneys. The corpuscles of Stannius and STC are considered to be an endocrine system that is unique to fishes. In this report, we provide evidence for the existence of STC-like proteins in vertebrates other than fishes, in particular, humans. By using a well-characterized RIA for salmon STC, sera from vertebrates as diverse as sharks and humans contained measurable levels of STC-like immunoreactivity in the concentration range commonly observed in fishes, and all of these sera exhibited parallelism in the assay. By using Western blot analysis, proteins were also identified in human kidney extracts that shared several properties with the fish hormone in addition to their cross-reactivity with salmon STC antiserum. The same antiserum was used to identify a discrete population of cells in human kidney tubules that could be the source of serum immunoreactivity. Human kidney extracts containing the STC-immunoreactive proteins also had STC-related effects when injected into fishes. Collectively, the data suggest that STC may be more widespread among the vertebrates than is currently accepted.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.92.6.1871