Chromatographic fingerprinting analysis of Zhizhu Wan preparation by high-performance liquid chromatography coupled with photodiode array detector

Traditional Chinese medicine (TCM) formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW), a classical Chines...

Full description

Saved in:
Bibliographic Details
Published in:Pharmacognosy Magazine 2014-10, Vol.10 (40), p.470-476
Main Authors: Sun, Hui, Chen, Xi, Zhang, Aihua, Sakurai, Tetsuro, Jiang, Jinzhong, Wang, Xijun
Format: Article
Language:English
Subjects:
Acids
Chinese medicine
Chromatography
Free radicals
Gastrointestinal diseases
Grants
High performance liquid chromatography
Medicine, Chinese
Methods
Original
Pharmaceutical industry
Pharmacology, Experimental
Science
Symmetry
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Traditional Chinese medicine (TCM) formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW), a classical Chinese medical formula, has been commonly used for the treatment of gastrointestinal disease, which pose a serious challenge to its quality control. In this work, a sensitive and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) was developed to control the quality of ZZW for chemical fingerprint analysis and quantitative analysis of four major bioactive constituents, including hesperidin, naringin, neohesperidin, and atractylenolide I. The chromatographic separation was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm particle size), with an aqueous 0.095% phosphate acid and acetonitrile mobile phase gradient. Optimization of other experimental conditions was validated with satisfactory accuracy, precision, repeatability, and recovery. In quantitative analysis, the four components showed good regression (R > 0.9994) within test ranges, and the recovery method ranged from 99.32% to 100.630%. HPLC fingerprints of the ZZW samples were compared by performing similarity analysis. The results indicated that the newly developed HPLC-PDA fingerprint method would be suitable for quality control of ZZW.
ISSN:0973-1296
0976-4062
DOI:10.4103/0973-1296.141819