Changes in levels of angiotensin II and its receptors in a model of inverted stress-induced cardiomyopathy

• Published in: European journal of medical research 2014-10, Vol.19 (1), p.54-54, Article 54
• Main Authors: Xi, Yin-yan, Liu, Bei, Yang, Li-xia, Kuang, Chen-wei, Guo, Rui-wei
• Format: Article
• Language: English
• Subjects: Angiotensin I - metabolism; Angiotensin II - metabolism; Animals; Cardiomyopathies - metabolism; Disease Models, Animal; Female; Myocardium - metabolism; Peptide Fragments - metabolism; Rabbits; Receptors, Angiotensin - metabolism; Renin-Angiotensin System - physiology; Stress, Physiological - physiology
• ISSN: 2047-783X; 0949-2321; 2047-783X
• DOI: 10.1186/s40001-014-0054-8

Stress-induced cardiomyopathy (SIC) has gained increasing attention worldwide and is characterized by extensive ventricular akinesis, Beta-blockers and angiotensin-converting enzyme inhibitors (ACEIs) are the main treatments for SIC patients. The pharmacological mechanism of action of beta-blockers results in the inhibition of the biological effects of catecholamines. However, the mechanism of action of ACEIs in the treatment of cardiomyopathy is not known. Our aim is to assess changes in levels of angiotensin II, angiotensin-II receptors and ACE responses to SIC. A model of inverted SIC was established in rabbits by vagal electrical stimulation. The serum concentration of angiotensin II and angiotensin (1-7) was detected by enzyme-linked immunosorbent assay. Expression of angiotensin-II receptors was measured by Western blotting and real-time reverse transcription-polymerase chain reaction (RT-PCR), with localization detected by immunofluorescent staining. ACE-II expression in the myocardium was measured by Western blotting. From one day after vagal stimulation, concentrations of angiotensin II were significantly higher in the experimental group than those in the control group (P