Macrophages are required for influenza virus infection of human lymphocytes

Monocyte and lymphocyte surface-expressed viral antigens have been demonstrated after exposure of unseparated human mononuclear leukocytes to influenza virus in vitro. The current studies, using [35S]methionine pulse-labeled purified preparations of virus-exposed macrophages, depleted of lymphocytes...

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Bibliographic Details
Published in:The Journal of clinical investigation 1987-02, Vol.79 (2), p.620-624
Main Authors: MOCK, D. J, DOMURAT, F, ROBERTS, N. J. JR, WALSH, E. E, LICHT, M. R, KENG, P
Format: Article
Language:English
Subjects:
Antigens, Viral - analysis
Biological and medical sciences
Cell Transformation, Viral
Fundamental and applied biological sciences. Psychology
Humans
influenza A virus
Influenza A virus - genetics
Influenza, Human - blood
Lymphocytes - physiology
Macrophages - physiology
Methionine - metabolism
Microbiology
Molecular Weight
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Sulfur Radioisotopes
Viral Proteins - biosynthesis
Virology
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Summary:Monocyte and lymphocyte surface-expressed viral antigens have been demonstrated after exposure of unseparated human mononuclear leukocytes to influenza virus in vitro. The current studies, using [35S]methionine pulse-labeled purified preparations of virus-exposed macrophages, depleted of lymphocytes, demonstrate that the presence of these viral proteins does represent new synthesis. However, purified lymphocytes, depleted of monocytes-macrophages and exposed to influenza virus, showed no detectable viral protein synthesis. In further experiments, unseparated mononuclear leukocytes were exposed to virus and subsequently separated by countercurrent centrifugal elutriation. Both macrophages and lymphocytes were then shown to synthesize influenza proteins. Cell-free control or influenza virus-infected macrophage-derived supernatant fluids did not facilitate influenza virus infection of the lymphocytes. The data suggest that macrophages are required for influenza virus infection of human lymphocytes, and raise the possibility that macrophage facilitation of an abortive infection of lymphocytes plays a role in the generation of effective immunity to viral antigens.
ISSN:0021-9738
1558-8238
DOI:10.1172/JCI112856