Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers

We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, expres...

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Bibliographic Details
Published in:The Journal of clinical investigation 1987-03, Vol.79 (3), p.859-866
Main Authors: PAPAYANNOPOULOU, T, RAINES, E, COLLINS, S, NAKAMOTO, B, TWEEDDALE, M, ROSS, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Differentiation
Cell Line
Erythrocytes - metabolism
Hematologic and hematopoietic diseases
Hematopoietic Stem Cells - metabolism
Humans
Kinetics
Leukemia, Erythroblastic, Acute - metabolism
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Medical sciences
Megakaryocytes - metabolism
Platelet Membrane Glycoproteins - metabolism
Platelet-Derived Growth Factor - genetics
Platelet-Derived Growth Factor - metabolism
RNA, Messenger - genetics
Tetradecanoylphorbol Acetate - pharmacology
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Summary:We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.
ISSN:0021-9738
1558-8238
DOI:10.1172/jci112895