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Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers
We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, expres...
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| Published in: | The Journal of clinical investigation 1987-03, Vol.79 (3), p.859-866 |
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| Main Authors: | , , , , , |
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| Language: | English |
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| container_end_page | 866 |
| container_issue | 3 |
| container_start_page | 859 |
| container_title | The Journal of clinical investigation |
| container_volume | 79 |
| creator | PAPAYANNOPOULOU, T RAINES, E COLLINS, S NAKAMOTO, B TWEEDDALE, M ROSS, R |
| description | We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells. |
| doi_str_mv | 10.1172/jci112895 |
| format | article |
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The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.</description><identifier>ISSN: 0021-9738</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/jci112895</identifier><identifier>PMID: 3469217</identifier><identifier>CODEN: JCINAO</identifier><language>eng</language><publisher>Ann Arbor, MI: American Society for Clinical Investigation</publisher><subject>Biological and medical sciences ; Cell Differentiation ; Cell Line ; Erythrocytes - metabolism ; Hematologic and hematopoietic diseases ; Hematopoietic Stem Cells - metabolism ; Humans ; Kinetics ; Leukemia, Erythroblastic, Acute - metabolism ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Medical sciences ; Megakaryocytes - metabolism ; Platelet Membrane Glycoproteins - metabolism ; Platelet-Derived Growth Factor - genetics ; Platelet-Derived Growth Factor - metabolism ; RNA, Messenger - genetics ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>The Journal of clinical investigation, 1987-03, Vol.79 (3), p.859-866</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-237cb0a675a4382b17c03bf544ad8e46bf99ca9de6923f2f3f233919f84074bd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC424221/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC424221/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8122557$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3469217$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PAPAYANNOPOULOU, T</creatorcontrib><creatorcontrib>RAINES, E</creatorcontrib><creatorcontrib>COLLINS, S</creatorcontrib><creatorcontrib>NAKAMOTO, B</creatorcontrib><creatorcontrib>TWEEDDALE, M</creatorcontrib><creatorcontrib>ROSS, R</creatorcontrib><title>Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.</description><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Erythrocytes - metabolism</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Leukemia, Erythroblastic, Acute - metabolism</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Medical sciences</subject><subject>Megakaryocytes - metabolism</subject><subject>Platelet Membrane Glycoproteins - metabolism</subject><subject>Platelet-Derived Growth Factor - genetics</subject><subject>Platelet-Derived Growth Factor - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>0021-9738</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpVkbuO1DAUhi0EWoaFggdAcoGQKALxLYkLCjTismglGqgjxznOeMexB9tZyMPwrniY0QiKo1P8338u-hF6Tuo3hLT07Z22hNBOigdoQ4Toqo6y7iHa1DUllWxZ9xg9Semurgnngl-hK8YbSUm7Qb-3wads85LtPWDlR2z9uGg7OMAJdIRsg8fB4INTGRzkaoRY0BFPMfzMO2yUziEWp3JhSnhY8W6ZlccOlj3MVmMNzmFnPSSsA_w6REjJ-glDXPMuBjv-3TrDpPYqrkGvuZhmFfcQ01P0yCiX4Nm5X6PvHz98236ubr9-utm-v600lyJXlLV6qFXTCsVZRwfS6poNRnCuxg54MxgptZIjlKeZoaYUY5JI0_G65cPIrtG709zDMswwavA5Ktcfoi2HrH1Qtv9f8XbXT-G-55RTSor_1dkfw48FUu5nm46PKw9hST3hjZANO4KvT6COIaUI5rKD1P0xyv7L9uYUZWFf_HvUhTxnV_SXZ10lrZyJymubLlhHKBWiZX8AZaqsFw</recordid><startdate>19870301</startdate><enddate>19870301</enddate><creator>PAPAYANNOPOULOU, T</creator><creator>RAINES, E</creator><creator>COLLINS, S</creator><creator>NAKAMOTO, B</creator><creator>TWEEDDALE, M</creator><creator>ROSS, R</creator><general>American Society for Clinical Investigation</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope><scope>5PM</scope></search><sort><creationdate>19870301</creationdate><title>Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers</title><author>PAPAYANNOPOULOU, T ; RAINES, E ; COLLINS, S ; NAKAMOTO, B ; TWEEDDALE, M ; ROSS, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-237cb0a675a4382b17c03bf544ad8e46bf99ca9de6923f2f3f233919f84074bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Erythrocytes - metabolism</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Leukemia, Erythroblastic, Acute - metabolism</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Medical sciences</topic><topic>Megakaryocytes - metabolism</topic><topic>Platelet Membrane Glycoproteins - metabolism</topic><topic>Platelet-Derived Growth Factor - genetics</topic><topic>Platelet-Derived Growth Factor - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PAPAYANNOPOULOU, T</creatorcontrib><creatorcontrib>RAINES, E</creatorcontrib><creatorcontrib>COLLINS, S</creatorcontrib><creatorcontrib>NAKAMOTO, B</creatorcontrib><creatorcontrib>TWEEDDALE, M</creatorcontrib><creatorcontrib>ROSS, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PAPAYANNOPOULOU, T</au><au>RAINES, E</au><au>COLLINS, S</au><au>NAKAMOTO, B</au><au>TWEEDDALE, M</au><au>ROSS, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1987-03-01</date><risdate>1987</risdate><volume>79</volume><issue>3</issue><spage>859</spage><epage>866</epage><pages>859-866</pages><issn>0021-9738</issn><eissn>1558-8238</eissn><coden>JCINAO</coden><abstract>We have examined the constitutive and inducible secretion of platelet-derived growth factor (PDGF)-like proteins in a variety of human hemopoietic cell lines. The highest levels of secreted protein were noted in four human erythroleukemia lines which, in addition to erythroid lineage markers, express one or more megakaryocytic lineage markers. Induction of these lines by 12-O-tetradecanoylphorbol-13-acetate enhanced the expression of megakaryocytic markers and increased secretion of PDGF-like proteins several fold. In concert with these changes, there was significant induction of c-sis/PDGF-B messenger RNA (mRNA) expression in all lines, whereas one line showed significant concurrent induction of PDGF-A mRNA expression. Whether PDGF-like secretion is part of the stem cell-like phenotype displayed by these lines or is secondary to their leukemic transformation remains to be determined. Nevertheless, these lines provide new cellular models for studying the expression and function of PDGF analogs in hemopoietic cells.</abstract><cop>Ann Arbor, MI</cop><pub>American Society for Clinical Investigation</pub><pmid>3469217</pmid><doi>10.1172/jci112895</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of clinical investigation, 1987-03, Vol.79 (3), p.859-866 |
| issn | 0021-9738 1558-8238 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_424221 |
| source | PubMed Central; EZB Electronic Journals Library |
| subjects | Biological and medical sciences Cell Differentiation Cell Line Erythrocytes - metabolism Hematologic and hematopoietic diseases Hematopoietic Stem Cells - metabolism Humans Kinetics Leukemia, Erythroblastic, Acute - metabolism Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Megakaryocytes - metabolism Platelet Membrane Glycoproteins - metabolism Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - metabolism RNA, Messenger - genetics Tetradecanoylphorbol Acetate - pharmacology |
| title | Constitutive and inducible secretion of platelet-derived growth factor analogs by human leukemic cell lines coexpressing erythroid and megakaryocytic markers |
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