Analysis of Nuclear DNA Content in Capsicum (Solanaceae) by Flow Cytometry and Feulgen Densitometry

Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare ‘Ditta’, 1C = 5·063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chine...

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Bibliographic Details
Published in:Annals of botany 2003-07, Vol.92 (1), p.21-29
Main Authors: MOSCONE, EDUARDO A., BARANYI, MONIKA, EBERT, IRMA, GREILHUBER, JOHANN, EHRENDORFER, FRIEDRICH, HUNZIKER, ARMANDO T.
Format: Article
Language:English
Subjects:
Biological taxonomies
Capsicum
Capsicum - genetics
Cell Nucleus - genetics
Chromosomes
Chromosomes, Plant - genetics
Coloring Agents - chemistry
Cytometry
Densitometry - methods
DNA
DNA, Plant - analysis
Evolution
Feulgen densitometry
flow cytometry
Flow Cytometry - methods
Genetic variation
Genome size
genome size variation
Genome, Plant
Heterochromatin
karyosystematics
Karyotype
karyotype evolution
Karyotyping
Key words: Solanaceae
Original
Plants
Rosaniline Dyes
Species Specificity
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Summary:Flow cytometric measurements of nuclear DNA content were performed using ethidium bromide as the DNA stain (internal standard, Hordeum vulgare ‘Ditta’, 1C = 5·063 pg) in 25 samples belonging to nine diploid species and four varieties of Capsicum: C. chacoense, C. parvifolium, C. frutescens, C. chinense, C. annuum var. annuum, C. baccatum var. baccatum, C. baccatum var. pendulum, C. baccatum var. umbilicatum, C. eximium and C. pubescens, all with 2n = 24, and C. campylopodium with 2n = 26. In addition, one sample each of C. annuum var. annuum and C. pubescens were also analysed using Feulgen densitometry (standard, Allium cepa ‘Stuttgarter Riesen’, 1C = 16·75 pg). Both staining methods resulted in very similar relative values. Genome size displays significant variation between but not within species (except in C. campylopodium), and contributes to their taxonomic grouping. 1C‐values range from 3·34–3·43 pg (3273–3361 Mbp) in C. chacoense and the C. annuum complex to 4·53–5·77 pg (4439–5655 Mbp) in C. campylopodium and C. parvifolium. The data obtained support conclusions on phylogenetic relationships in the genus derived from karyotype analyses using chromosome banding approaches. In Capsicum, constitutive heterochromatin amount is correlated with genome size, except in C. parvifolium, and is regarded as an additive genomic component.
ISSN:0305-7364
1095-8290
DOI:10.1093/aob/mcg105