CCL20/MIP-3 alpha mRNA expression in the conjunctival epithelium of normal individuals and patients with vernal keratoconjunctivitis

Background CCL20, the single chemokine ligand for CCR6, contributes to recruiting CCR6-expressing memory B cells, memory T cells, Th17 cells and dendritic cells, and is involved in regulating immune responses, homeostasis, and inflammation in mucosal tissues. Methods CCL20 messenger RNA (mRNA) expre...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 2014-12, Vol.252 (12), p.1977-1984
Main Authors: Inada, Noriko, Ishimori, Akiko, Shoji, Jun
Format: Article
Language:English
Subjects:
Adolescent
Adult
Cells, Cultured
Chemokine CCL20 - genetics
Chemokine CCL24 - genetics
Child
Conjunctiva - metabolism
Conjunctivitis, Allergic - genetics
Epithelial Cells - metabolism
Female
Gene Expression Regulation - physiology
Healthy Volunteers
Humans
Inflammatory Disorders
Interleukin-8 - genetics
Male
Medicine
Medicine & Public Health
Ophthalmology
Real-Time Polymerase Chain Reaction
RNA, Messenger - genetics
Young Adult
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Summary:Background CCL20, the single chemokine ligand for CCR6, contributes to recruiting CCR6-expressing memory B cells, memory T cells, Th17 cells and dendritic cells, and is involved in regulating immune responses, homeostasis, and inflammation in mucosal tissues. Methods CCL20 messenger RNA (mRNA) expression was analyzed in the conjunctival epithelium in an in vivo study of patients with vernal keratoconjunctivitis (VKC group) and healthy volunteers (control group) using impression cytology. In vitro analysis of CCL20 mRNA was performed using cultured conjunctival epithelial cells (CECs). Real-time polymerase chain reaction was used to assess IL-8 and eotaxin-2 mRNA expression for comparison with CCL20 mRNA expression. Results In the control group, CCL20 mRNA expression was present in all conjunctival locations. However, CCL20 mRNA expression was significantly higher in the upper palpebral conjunctiva in the severe VKC group than in the mild VKC and control groups ( p  
ISSN:0721-832X
1435-702X
DOI:10.1007/s00417-014-2785-1