The impact of vitrification on murine germinal vesicle oocyte In vitro maturation and aurora kinase A protein expression

Purpose Investigate the effect of vitrification on in vitro maturation (IVM) and expression of Aurora kinases A, B, and C in germinal vesicle (GV)-stage oocytes. Methods GV-stage oocytes from B6D2F1 female mice 7–11 weeks of age were vitrified after collection, thawed, and matured in vitro for 0, 4,...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2014-12, Vol.31 (12), p.1695-1702
Main Authors: Doyle, Joseph O., Lee, Ho Joon, Selesniemi, Kaisa, Styer, Aaron K., Rueda, Bo R.
Format: Article
Language:English
Subjects:
Animals
Aurora Kinase A - biosynthesis
Aurora Kinase A - genetics
Biology
Blastocyst - physiology
Cell cycle
Chromosomes
Cleavage Stage, Ovum
Cryopreservation
Eggs
Embryos
Female
Fertilization in Vitro
Gamete Biology
Gene Expression Regulation
Gynecology
Human Genetics
Humans
In Vitro Oocyte Maturation Techniques
Kinases
Medicine
Medicine & Public Health
Meiosis - genetics
Mice
Obstetrics
Oogenesis - genetics
Oogenesis - physiology
Protein expression
Proteins
Reproductive Medicine
Vitrification
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Summary:Purpose Investigate the effect of vitrification on in vitro maturation (IVM) and expression of Aurora kinases A, B, and C in germinal vesicle (GV)-stage oocytes. Methods GV-stage oocytes from B6D2F1 female mice 7–11 weeks of age were vitrified after collection, thawed, and matured in vitro for 0, 4, 8, and 12 h (hrs). The rate of germinal vesicle breakdown (GVBD), spindle apparatus assembly, and Aurora kinase mRNA and protein expression during IVM was measured. Results Oocyte vitrification was associated with significant delays in both GVBD and normal spindle apparatus assembly at 4 and 8 h of IVM ( p  
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-014-0336-7