Purification and functional characterization of pancreatic insulin from camel (Camelus dromedarius)

Large-scale production of insulin still represents the key step in helping diabetic patients throughout the world. Many species and approaches have been used for the production of insulin. In this study, we purified and characterized for the first time pancreatic insulin from the Arabian camel (Came...

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Bibliographic Details
Published in:Saudi journal of biological sciences 2014-12, Vol.21 (6), p.574-581
Main Authors: Elamin, Babiker A., Al-Maleki, Abdulmajeed, Ismael, Mohammad A., Ayoub, Mohammed Akli
Format: Article
Language:English
Subjects:
Acid-alcohol extraction
amino acid composition
Biotechnology
Camels
Camelus dromedarius
cattle
Chromatographic analysis
Chromatography
economic valuation
gel chromatography
high performance liquid chromatography
humans
hypoglycemia
Insulin
mice
molecular weight
Original
pancreas
patients
Physiology
polyacrylamide gel electrophoresis
radioimmunoassays
swine
الإبل
الإنسولين
التكنولوجيا الحيوية
الفسيولوجيا
الكيمياء التحليلية
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Summary:Large-scale production of insulin still represents the key step in helping diabetic patients throughout the world. Many species and approaches have been used for the production of insulin. In this study, we purified and characterized for the first time pancreatic insulin from the Arabian camel (Camelus dromedarius) using a modified acid-alcohol extraction method. After extraction insulin was purified using a one-step gel filtration on a Sephadex G-50 column leading to a purification yield of 80mg/kg (20%) of camel pancreas. The purity of camel insulin was assessed by SDS–PAGE and HPLC using insulin from human, bovine and porcine as standards. Molecular weight was determined for purified camel insulin as 5800 Daltons and its amino acid composition is similar to that known for other species. The functional characterization of purified crude camel insulin was demonstrated in vitro by positive competition by radioimmunoassay and in vivo showing camel insulin inducing acute hypoglycaemia in mice. Together, our study reports for the first time the successful purification of functional insulin from camel pancreas with similar properties compared to other insulin species. This is of great interest given that the camel represents considerable economic worth in many countries.
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2014.03.001