Application of response surface method to evaluate the cytotoxic potency of Ulva fasciata Delile, a marine macro alga

•Cytotoxicity of Ulva fasciata Delile; a marine macro alga was revealed for the first time.•In vitro cytotoxicity was carried out on HT-29, Hep-G2 and MCF-7.•Response surface methodology was employed to optimize the complete study. Bioprospecting of marine natural products has recently produced a su...

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Bibliographic Details
Published in:Saudi journal of biological sciences 2014-12, Vol.21 (6), p.539-546
Main Authors: Das, Mukesh Kumar, Sahu, Prafulla Kumar, Rao, G. Srinivasa, Mukkanti, K., Silpavathi, L.
Format: Article
Language:English
Subjects:
absorbance
Algae
breast neoplasms
Cancer cells
carcinoma
cell viability
Cell-mediated cytotoxicity
colorectal neoplasms
Cytotoxicity
drugs
experimental design
Extracts
Green marine macro alga
humans
India
MTT assay
neoplasm cells
Original
Response surface methodology
tetrazolium
Ulva
Ulva fasciata Delile
الخلايا السرطانية
الطحالب
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Summary:•Cytotoxicity of Ulva fasciata Delile; a marine macro alga was revealed for the first time.•In vitro cytotoxicity was carried out on HT-29, Hep-G2 and MCF-7.•Response surface methodology was employed to optimize the complete study. Bioprospecting of marine natural products has recently produced a substantial number of drug candidates. Ulva fasciata Delile, belonging to the family Ulvaceae, is a green marine macro alga that grows profusely on the coastal seashore of South India. In the present study, we investigated the in vitro cytotoxic potential of a methanolic extract of U.fasciata Delile (MEUF) using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay against human colon carcinoma (HT-29), human hepatocyte carcinoma (Hep-G2), and human breast carcinoma (MCF-7) cell lines. Response surface methodology (RSM) was applied using central-composite experimental design (CCD) to obtain optimum combined effect of concentration and cancer cells with highest cytotoxicity. The effect of concentration, cancer cell lines as independent variables on absorbance (OD), percent cell survival and percent cell inhibition as dependent variables was investigated. Maximum cytotoxic activity of MEUF was established for Hep-G2 with lowest OD or percent cell survival; highest percent cell inhibition with significant difference (p>0.05) was compared to HT-29 and MCF-7.
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2014.02.003