Expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis

• Published in: World journal of gastroenterology : WJG 2005-01, Vol.11 (4), p.529-533
• Main Authors: Song, Pei-Song, Kong, Kang-Mei, Niu, Chun-Yan, Qi, Wei-Li, Wu, Ling-Fei, Wang, Xin-Jia, Han, Wei, Huang, Keng, Chen, Ze-Feng
• Format: Article
• Language: English
• Subjects: Animals; Basic Research; c-fos; Cervical Vertebrae; Female; Gastrointestinal Diseases - etiology; Male; Myenteric Plexus - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Pyloric Antrum - innervation; Pyloric Antrum - physiology; Rats; Rats, Sprague-Dawley; Spinal Cord - metabolism; Spinal Osteophytosis - complications; Spinal Osteophytosis - metabolism; Spinal Osteophytosis - physiopathology; 基因表达; 椎关节强硬; 胃肠肌层血管
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i4.529

AIM: To determine the expression of c-fos in gastric myenteric plexus and spinal cord of rats with cervical spondylosis and its clinical significance.METHODS: A cervical spondylosis model was established in rats by destroying the stability of cervical posterior column,and bhe cord segments C4-6 and gastric antrum were collected 3, 4 and 5 mo after the operation. Rats with sham operation were used as controls, c-fos neuronal counter-staining was performed with an immunohistochemistry method. Every third sections from C4-6 segments were drawn. The 10most labeled c-fos-immunoreactive (Fos-IR) neurons were counted, and the average number was used for statistical analysis. The mean of Fos-IR neurons in myenteric plexus was calculated after counting Fos-IR neurons in 25 ganglia from each antral preparation, and expressed as a mean count per myenteric ganglion.RESULTS: There were a few c-fos-positive neurons in the cervical cord and antrum in the control group. There was an increased c-fos expression in model group 3, 4 and 5 mo after operation, whereas there was no significant increase in c-fos expression in the control group at 3, 4 and 5 mo.More importantly, there was a significant difference in c-fos expression between rats followed up for 3 mo and those for 5 mo in the model group (11.20±2.26 vs 27.68±4.36,P