Evaluating the effect of assay preparation on the uptake of gold nanoparticles by RAW264.7 cells

Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10 nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to de...

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Bibliographic Details
Published in:Journal of nanobiotechnology 2014-11, Vol.12 (1), p.45-45, Article 45
Main Authors: Bancos, Simona, Tyner, Katherine M
Format: Article
Language:English
Subjects:
Animals
Assaying
BASIC BIOLOGICAL SCIENCES
Biotechnology
Biotechnology & Applied Microbiology
Cell Culture Techniques
Cell Proliferation - drug effects
Comparative analysis
Culture
Dosimetry
Dynamic tests
Dynamics
Gold
Gold - chemistry
Gold - pharmacokinetics
Gold nanoparticle
In vitro assay
Laboratories
Limit of Detection
Macrophage
Mice
Microscopy
Nanomaterials
Nanoparticle dosimetry
Nanoparticle uptake
Nanoparticles
Nanoparticles - chemistry
Neutron Activation Analysis
Physiological aspects
RAW 264.7 Cells - drug effects
Science & Technology - Other Topics
Studies
Uptakes
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Summary:Cell culture conditions can greatly influence the results of nanoparticle (NP) uptake assays. In this study, 10 nm gold nanoparticles (AuNPs) and RAW 264.7 macrophages were used as a model system, while instrumental neutron activation analysis (NAA) was used as the elemental analysis technique to determine AuNP levels produced by the various culturing conditions. Static plate-based and insert-based culture conditions were compared with a dynamic suspension culture to evaluate the conditions' effect on the rate and extent of AuNP uptake. The results indicate that a dynamic culturing condition allows for the greatest NP uptake (approximately 3-5 times over the adherent conditions), whereas the plate-based assays have the initial highest rate of NP incorporation. These data highlight the importance of judiciously choosing the assay conditions prior to evaluating NP uptake.
ISSN:1477-3155
1477-3155
DOI:10.1186/s12951-014-0045-5