Methionine protects against hyperthermia-induced cell injury in cultured bovine mammary epithelial cells

• Published in: Cell stress & chaperones 2015-01, Vol.20 (1), p.109-120
• Main Authors: Han, Zhao-Yu, Mu, Tian, Yang, Zhen
• Format: Article
• Language: English
• Subjects: Animals; Annexins; Apoptosis; Apoptosis - drug effects; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cancer Research; Caspase 3 - metabolism; Cattle; Cell Biology; Cell Survival - drug effects; Cells, Cultured; Cultured cells; Dairy cattle; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Cells - pathology; Female; Heat Shock Transcription Factors; Heat stress disorders; HSP70 Heat-Shock Proteins - genetics; HSP70 Heat-Shock Proteins - metabolism; Hyperthermia; Hyperthermia, Induced; Immunology; L-Lactate Dehydrogenase - metabolism; Mammary Glands, Animal - cytology; Mammary Glands, Animal - drug effects; Messenger RNA; Methionine - pharmacology; Neurosciences; Nitric Oxide - analysis; Original Paper; Oxidoreductases - metabolism; Protein synthesis; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA, Messenger - metabolism; Temperature; Transcription Factors - genetics; Transcription Factors - metabolism; Ungulates
• ISSN: 1355-8145; 1466-1268
• DOI: 10.1007/s12192-014-0530-7

The aim of this study was to investigate the effects of methionine on cell proliferation, antioxidant activity, apoptosis, the expression levels of related genes (HSF-1, HSP70, Bax and Bcl-2) and the expression levels of protein (HSP70) in mammary epithelial cells, after heat treatment. Methionine (60 mg/L) increased the viability and attenuated morphological damage in hyperthermia-treated bovine mammary epithelial cells (BMECs). Additionally, methionine significantly reduced lactate dehydrogenase leakage, malondialdehyde formation, nitric oxide, and nitric oxide synthase activity. Superoxide dismutase, catalase, and glutathione peroxidase enzymatic activity was increased significantly in the presence of methionine. Bovine mammary epithelial cells also exhibited a certain amount of HSP70 reserve after methionine pretreatment for 24 h, and the expression level of the HSP70 gene and protein further increased with incubation at 42 °C for 30 min. Compared to the control, the expression of HSF-1 mRNA increased, and there was a significantly reduced expression of Bax/Bcl-2 mRNA and a reduced activity of caspase-3 against heat stress. Methionine also increased survival and decreased early apoptosis of hyperthermia-treated BMECs. Thus, methionine has cytoprotective effects on hyperthermia-induced damage in BMECs.