Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins

Abstract To modulate the surface properties of collagen and subsequent cell–surface interactions, a method was developed to transfer protein patterns from glass coverslips to collagen type I hydrogel surfaces. Two proteins and one proteoglycan found in central nervous system extracellular matrix as...

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Bibliographic Details
Published in:Biomaterials 2015-01, Vol.39, p.124-130
Main Authors: Hsiao, Tony W, Tresco, Patrick A, Hlady, Vladimir
Format: Article
Language:English
Subjects:
Advanced Basic Science
Alignment
Animals
Astrocytes
Astrocytes - cytology
Astrocytes - physiology
Cells, Cultured
Cellular
Central nervous system
Chondroitin sulfate proteoglycan
Chondroitin Sulfate Proteoglycans - chemistry
Collagen - chemistry
Collagen I
Collagens
Dentistry
ECM
Extracellular Matrix Proteins - chemistry
Glass
Hydrogels
Hydrogels - chemistry
Immunohistochemistry
Microcontact printing
Proteins
Rats
Sulfates
Surface patterns
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Summary:Abstract To modulate the surface properties of collagen and subsequent cell–surface interactions, a method was developed to transfer protein patterns from glass coverslips to collagen type I hydrogel surfaces. Two proteins and one proteoglycan found in central nervous system extracellular matrix as well as fibrinogen were patterned in stripes onto collagen hydrogel and astrocytes were cultured on these surfaces. The addition of the stripe protein patterns to hydrogels created astrocyte layers in which cells were aligned with underlying patterns and had reduced chondroitin sulfate expression compared to the cells grown on collagen alone. Protein patterns were covalently cross-linked to the collagen and stable over four days in culture with no visible cellular modifications. The present method can be adapted to transfer other types of protein patterns from glass coverslips to collagen hydrogels.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2014.10.062