Use of Tetraethylthiuram Disulfide to Discriminate between Alternative Respiration and Lipoxygenase 1

Mitochondria from axes of Glycine max (L.) Merr. cv. Chippewa 64 seedlings purified on discontinuous Percoll gradients exhibited classical cyanide-resistant respiration. These mitochondria also possessed lipoxygenase activity, as determined by O 2 uptake in the presence of 0.8 millimolar linoleic ac...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1981-05, Vol.67 (5), p.962-964
Main Authors: Miller, Marna Geyer, Obendorf, Ralph L.
Format: Article
Language:English
Online Access:Get full text
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Summary:Mitochondria from axes of Glycine max (L.) Merr. cv. Chippewa 64 seedlings purified on discontinuous Percoll gradients exhibited classical cyanide-resistant respiration. These mitochondria also possessed lipoxygenase activity, as determined by O 2 uptake in the presence of 0.8 millimolar linoleic acid. This activity is inhibited by most known inhibitors of alternative respiration ( i.e. hydroxamates and propyl gallate). Tetraethylthiuram disulfide (disulfiram) at 50 micromolar inhibited cyanide-resistant succinate oxidation by 90 per cent, whereas concentrations as high as 100 micromolar had no effect on lipoxygenase activity. Use of tetraethylthiuram disulfide allows discrimination between alternative respiration and lipoxygenase activity in mitochondria.
ISSN:0032-0889
1532-2548