Stem Cells of the Apical Papilla Regulate Trigeminal Neurite Outgrowth and Targeting through a BDNF-Dependent Mechanism

Regenerative endodontic procedures have become a valuable alternative for the treatment of immature teeth with pulp necrosis. In addition to resolution of periradicular pathosis and promotion of continued root development, positive vitality testing has been observed in some regenerative clinical cas...

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Bibliographic Details
Published in:Tissue engineering. Part A 2014-12, Vol.20 (23-24), p.389-3100
Main Authors: de Almeida, Jose Flavio A., Chen, Paul, Henry, Michael A., Diogenes, Anibal
Format: Article
Language:English
Subjects:
Animals
Brain-Derived Neurotrophic Factor - metabolism
Glial Cell Line-Derived Neurotrophic Factor - metabolism
Male
Mice, SCID
Nerve Growth Factor - metabolism
Neurites - metabolism
Neurons
Original
Original Articles
Rats
Rats, Sprague-Dawley
Rodents
Stem cells
Stem Cells - metabolism
Tissue engineering
Trigeminal Nerve - cytology
Trigeminal Nerve - metabolism
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Summary:Regenerative endodontic procedures have become a valuable alternative for the treatment of immature teeth with pulp necrosis. In addition to resolution of periradicular pathosis and promotion of continued root development, positive vitality testing has been observed in some regenerative clinical cases. Importantly, the positive response to electric stimulation of the regenerated tissue requires targeting of periradicular axons into the previously empty root canal space. However, the mechanism by which this process occurs is largely unknown. Since stem cells of the apical papilla (SCAP) have been proposed to populate the root canal following regenerative endodontic procedures, we hypothesized that SCAP regulate neurite outgrowth and axonal targeting. To test this hypothesis, we established primary co-cultures of human SCAP and rat trigeminal neurons, and performed neurite outgrowth assays using ELISA and confocal microscopy to determine the effect of increasing concentration of SCAP on the total neurite outgrowth and axonal targeting. In addition, we evaluated whether SCAP evoked axonal targeting in vivo using a matrigel subcutaneous implant assay. Data were analyzed by ANOVA with Bonferroni's post hoc test, and significance was set at p
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2013.0347