An Integrated Microfluidic Chip System for Single-Cell Secretion Profiling of Rare Circulating Tumor Cells

Genetic and transcriptional profiling, as well as surface marker identification of single circulating tumor cells (CTCs) have been demonstrated. However, quantitatively profiling of functional proteins at single CTC resolution has not yet been achieved, owing to the limited purity of the isolated CT...

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Bibliographic Details
Published in:Scientific reports 2014-12, Vol.4 (1), p.7499-7499, Article 7499
Main Authors: Deng, Yuliang, Zhang, Yu, Sun, Shuai, Wang, Zhihua, Wang, Minjiao, Yu, Beiqin, Czajkowsky, Daniel M., Liu, Bingya, Li, Yan, Wei, Wei, Shi, Qihui
Format: Article
Language:English
Subjects:
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Aged
Antibodies
Antigens, Neoplasm - metabolism
Biomarkers, Tumor - secretion
Blood
Blood cells
Cancer
Carcinoma, Squamous Cell - blood
Carcinoma, Squamous Cell - secondary
Case-Control Studies
Cell Adhesion Molecules - metabolism
Cell Separation - methods
Dietary Proteins - metabolism
Epithelial Cell Adhesion Molecule
Female
HCT116 Cells
Humanities and Social Sciences
Humans
Interleukin 8
Laboratories
Lung Neoplasms - blood
Lung Neoplasms - pathology
Lysine
Male
Medical screening
Metastasis
Microfluidics
Microfluidics - methods
multidisciplinary
Neoplasm Staging
Neoplastic Cells, Circulating - pathology
Poly-L-lysine
Population
Proteins
Proteomics
Proteomics - methods
Science
Single-Cell Analysis - methods
Surface markers
Transcription
Tumor cells
Vascular endothelial growth factor
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Summary:Genetic and transcriptional profiling, as well as surface marker identification of single circulating tumor cells (CTCs) have been demonstrated. However, quantitatively profiling of functional proteins at single CTC resolution has not yet been achieved, owing to the limited purity of the isolated CTC populations and a lack of single-cell proteomic approaches to handle and analyze rare CTCs. Here, we develop an integrated microfluidic system specifically designed for streamlining isolation, purification and single-cell secretomic profiling of CTCs from whole blood. Key to this platform is the use of photocleavable ssDNA-encoded antibody conjugates to enable a highly purified CTC population with
ISSN:2045-2322
2045-2322
DOI:10.1038/srep07499