Diesel biodegradation capacities of indigenous bacterial species isolated from diesel contaminated soil

Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study we isolated the bacteria from diesel conta...

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Bibliographic Details
Published in:Journal of environmental health science and engineering 2014-12, Vol.12 (1), p.142-142, Article 142
Main Authors: Palanisamy, Nandhini, Ramya, Jayaprakash, Kumar, Srilakshman, Vasanthi, NS, Chandran, Preethy, Khan, Sudheer
Format: Article
Language:English
Subjects:
Analysis
Biodegradation
Bioremediation
Carbon
Earth and Environmental Science
Environment
Environmental Economics
Environmental Engineering/Biotechnology
Environmental Health
Environmental Law/Policy/Ecojustice
Health aspects
Hydrocarbons
Microorganisms
Nitrogen
Oil and gas exploration
Quality of Life Research
Research Article
RNA
Soil contamination
Soil microbiology
Surface active agents
Surfactants
Waste Management/Waste Technology
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Summary:Petroleum based products are the major source of energy for industries and daily life. Leaks and accidental spills occur regularly during the exploration, production, refining, transport, and storage of petroleum and petroleum products. In the present study we isolated the bacteria from diesel contaminated soil and screened them for diesel biodegradation capacity. One monoculture isolate identified by 16S rRNA gene sequence analysis to be Acinetobacter baumannii was further studied for diesel oil biodegradation. The effects of various culture parameters (pH, temperature, NaCl concentrations, initial hydrocarbon concentration, initial inoculum size, role of chemical surfactant, and role of carbon and nitrogen sources) on biodegradation of diesel oil were evaluated. Optimal diesel oil biodegradation by A. baumanii occurred at initial pH 7, 35°C and initial hydrocarbon concentration at 4%. The biodegradation products under optimal cultural conditions were analyzed by GC-MS. The present study suggests that A. baumannii can be used for effective degradation of diesel oil from industrial effluents contaminated with diesel oil.
ISSN:2052-336X
2052-336X
DOI:10.1186/s40201-014-0142-2