Acetate transiently inhibits myocardial contraction by increasing mitochondrial calcium uptake

• Published in: BMC physiology 2014-12, Vol.14 (1), p.12-12, Article 12
• Main Authors: Schooley, James F, Namboodiri, Aryan M A, Cox, Rachel T, Bünger, Rolf, Flagg, Thomas P
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Anatomy & physiology; Animals; Apoptosis; Calcium; Calcium - metabolism; Cardiovascular diseases; Chemical properties; Experiments; Fatty acids; Glucose; Health sciences; Heart; Laboratory animals; Male; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria; Mitochondria - drug effects; Mitochondria - metabolism; Myocardial Contraction - drug effects; Physiological aspects; Rodents; Sodium Acetate - metabolism; Sodium Acetate - pharmacology; Studies
• ISSN: 1472-6793; 1472-6793
• DOI: 10.1186/s12899-014-0012-2

There is a close relationship between cardiovascular disease and cardiac energy metabolism, and we have previously demonstrated that palmitate inhibits myocyte contraction by increasing Kv channel activity and decreasing the action potential duration. Glucose and long chain fatty acids are the major fuel sources supporting cardiac function; however, cardiac myocytes can utilize a variety of substrates for energy generation, and previous studies demonstrate the acetate is rapidly taken up and oxidized by the heart. In this study, we tested the effects of acetate on contractile function of isolated mouse ventricular myocytes. Acute exposure of myocytes to 10 mM sodium acetate caused a marked, but transient, decrease in systolic sarcomere shortening (1.49 ± 0.20% vs. 5.58 ± 0.49% in control), accompanied by a significant increase in diastolic sarcomere length (1.81 ± 0.01 μm vs. 1.77 ± 0.01 μm in control), with a near linear dose response in the 1-10 mM range. Unlike palmitate, acetate caused no change in action potential duration; however, acetate markedly increased mitochondrial Ca(2+) uptake. Moreover, pretreatment of cells with the mitochondrial Ca(2+) uptake blocker, Ru-360 (10 μM), markedly suppressed the effect of acetate on contraction. Lehninger and others have previously demonstrated that the anions of weak aliphatic acids such as acetate stimulate Ca(2+) uptake in isolated mitochondria. Here we show that this effect of acetate appears to extend to isolated cardiac myocytes where it transiently modulates cell contraction.