Rapid Identification of Bacterial Biofilms and Biofilm Wound Models Using a Multichannel Nanosensor

Identification of infectious bacteria responsible for biofilm-associated infections is challenging due to the complex and heterogeneous biofilm matrix. To address this issue and minimize the impact of heterogeneity on biofilm identification, we developed a gold nanoparticle (AuNP)-based multichannel...

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Bibliographic Details
Published in:ACS nano 2014-12, Vol.8 (12), p.12014-12019
Main Authors: Li, Xiaoning, Kong, Hao, Mout, Rubul, Saha, Krishnendu, Moyano, Daniel F, Robinson, Sandra M, Rana, Subinoy, Zhang, Xinrong, Riley, Margaret A, Rotello, Vincent M
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biofilms
Biosensors
Gold
Gold - chemistry
Heterogeneity
Luminescent Proteins - chemistry
Metal Nanoparticles - chemistry
Mice
Multichannel
Nanostructure
Nanotechnology - instrumentation
NIH 3T3 Cells
Sensors
Time Factors
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Summary:Identification of infectious bacteria responsible for biofilm-associated infections is challenging due to the complex and heterogeneous biofilm matrix. To address this issue and minimize the impact of heterogeneity on biofilm identification, we developed a gold nanoparticle (AuNP)-based multichannel sensor to detect and identify biofilms based on their physicochemical properties. Our results showed that the sensor can discriminate six bacterial biofilms including two composed of uropathogenic bacteria. The capability of the sensor was further demonstrated through discrimination of biofilms in a mixed bacteria/mammalian cell in vitro wound model.
ISSN:1936-0851
1936-086X
DOI:10.1021/nn505753s