Calcium‐sensing receptor silencing in colorectal cancer is associated with promoter hypermethylation and loss of acetylation on histone 3

The calcium‐sensing receptor (CaSR) is suggested to mediate the antiproliferative effects of calcium in colon. However, in colorectal cancer (CRC) the expression of the CaSR is silenced and the underlying mechanisms leading to its loss are poorly understood. We investigated whether loss of the CaSR...

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Bibliographic Details
Published in:International journal of cancer 2014-11, Vol.135 (9), p.2014-2023
Main Authors: Fetahu, Irfete S., Höbaus, Julia, Aggarwal, Abhishek, Hummel, Doris M., Tennakoon, Samawansha, Mesteri, Ildiko, Baumgartner‐Parzer, Sabina, Kállay, Enikő
Format: Article
Language:English
Subjects:
Acetylation
Aged
Apoptosis - drug effects
Biological and medical sciences
Blotting, Western
Calcium
calcium‐sensing receptor
Cancer
Carcinogenesis
Cell Proliferation - drug effects
Chromatin Immunoprecipitation
Colorectal cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
DNA Methylation
Female
Fluorescent Antibody Technique
Gastroenterology. Liver. Pancreas. Abdomen
Gene Silencing
Genes
histone acetylation
Histone Deacetylase Inhibitors - pharmacology
histone methylation
Histones - metabolism
Humans
Hydroxamic Acids - pharmacology
Male
Medical research
Medical sciences
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neoplasm Grading
Neoplasm Staging
Prognosis
Promoter Regions, Genetic - genetics
Real-Time Polymerase Chain Reaction
Receptors, Calcium-Sensing - genetics
Receptors, Calcium-Sensing - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumor Cells, Cultured
Tumors
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Description
Summary:The calcium‐sensing receptor (CaSR) is suggested to mediate the antiproliferative effects of calcium in colon. However, in colorectal cancer (CRC) the expression of the CaSR is silenced and the underlying mechanisms leading to its loss are poorly understood. We investigated whether loss of the CaSR expression in colorectal tumors is caused by DNA hypermethylation and imbalance of transcriptionally permissive/repressive histone alterations. We observed significantly lower CaSR mRNA expression (n = 65, p 
ISSN:0020-7136
1097-0215
DOI:10.1002/ijc.28856