Bacterial community dynamics during the early stages of biofilm formation in a chlorinated experimental drinking water distribution system: implications for drinking water discolouration

AIMS: To characterize bacterial communities during the early stages of biofilm formation and their role in water discolouration in a fully representative, chlorinated, experimental drinking water distribution systems (DWDS). METHODS AND RESULTS: Biofilm development was monitored in an experimental D...

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Bibliographic Details
Published in:Journal of applied microbiology 2014-07, Vol.117 (1), p.286-301
Main Authors: Douterelo, I, Sharpe, R, Boxall, J
Format: Article
Language:English
Subjects:
16s rRNA sequencing
Bacteria
bacterial communities
bacterial community structure
Biodiversity
biofilm
biofilm development
Biofilms
Biofilms - growth & development
Biological and medical sciences
cleaning
community structure
discoloration
discolouration
Drinking water
Drinking Water - microbiology
drinking water distribution systems
fluorescence microscopy
Fundamental and applied biological sciences. Psychology
genes
Genes, rRNA
Halogenation
Janthinobacterium
Microbial Consortia - genetics
Microbiology
Original
Oxalobacteraceae - classification
Oxalobacteraceae - genetics
Phylogeny
Pseudomonas
Pseudomonas - classification
Pseudomonas - genetics
ribosomal RNA
risk
RNA, Ribosomal, 16S - genetics
terminal restriction fragment length polymorphism
water distribution
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Summary:AIMS: To characterize bacterial communities during the early stages of biofilm formation and their role in water discolouration in a fully representative, chlorinated, experimental drinking water distribution systems (DWDS). METHODS AND RESULTS: Biofilm development was monitored in an experimental DWDS over 28 days; subsequently the system was disturbed by raising hydraulic conditions to simulate pipe burst, cleaning or other system conditions. Biofilm cell cover was monitored by fluorescent microscopy and a fingerprinting technique used to assess changes in bacterial community. Selected samples were analysed by cloning and sequencing of the 16S rRNA gene. Fingerprinting analysis revealed significant changes in the bacterial community structure over time (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12516