Beneficial effect of an omega-6 PUFA-rich diet in non-steroidal anti-inflammatory drug-induced mucosal damage in the murine small intestine

• Published in: World journal of gastroenterology : WJG 2015-01, Vol.21 (1), p.177-186
• Main Authors: Ueda, Toshihide, Hokari, Ryota, Higashiyama, Masaaki, Yasutake, Yuichi, Maruta, Koji, Kurihara, Chie, Tomita, Kengo, Komoto, Shunsuke, Okada, Yoshikiyo, Watanabe, Chikako, Usui, Shingo, Nagao, Shigeaki, Miura, Soichiro
• Format: Article
• Language: English
• Subjects: Animals; anti-inflammatory; Anti-Inflammatory Agents, Non-Steroidal - toxicity; Basic Study; Blood Platelets - drug effects; Blood Platelets - immunology; Blood Platelets - metabolism; Cell Adhesion - drug effects; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Chemotaxis, Leukocyte - drug effects; Diet; drugs; Small; Fatty Acids, Omega-6 - administration & dosage; Fish Oils - administration & dosage; Fish Oils - toxicity; Indomethacin - toxicity; intest; Intestinal Mucosa - blood supply; Intestinal Mucosa - drug effects; Intestinal Mucosa - immunology; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Intestine, Small - blood supply; Intestine, Small - drug effects; Intestine, Small - immunology; Intestine, Small - metabolism; Intestine, Small - pathology; Leukocytes - drug effects; Leukocytes - immunology; Leukocytes - metabolism; Male; Meat Products - toxicity; Mice, Inbred C57BL; Microvessels - drug effects; Microvessels - immunology; Microvessels - metabolism; Non-steroidal; RNA, Messenger - metabolism; Safflower Oil - administration & dosage; Time Factors
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v21.i1.177

AIM: To investigate the effect of a fat rich diet onnon-steroidal anti-inflammatory drug(NSAID)-induced mucosal damage in the murine small intestine.METHODS: C57BL6 mice were fed 4 types of diets with or without indomethacin.One group was fed standard laboratory chow.The other groups were fed a fat diet consisting of 8% w/w fat,beef tallow(rich in SFA),fish oil,(rich in omega-3 PUFA),or safflower oil(rich in omega-6 PUFA).Indomethacin(3 mg/kg) was injected intraperitoneally from day 8 to day 10.On day 11,intestines and adhesions to submucosal microvessels were examined.RESULTS: In the indomethacin-treated groups,mucosal damage was exacerbated by diets containing beef tallow and fish oil,and was accompanied by leukocyte infiltration(P < 0.05).The mucosal damage induced by indomethacin was significantly lower in mice fed the safflower oil diet than in mice fed the beef tallow or fish oil diet(P < 0.05).Indomethacin increased monocyte and platelet migration to the intestinal mucosa,whereas safflower oil significantly decreased monocyte and platelet recruitment(P < 0.05).CONCLUSION: A diet rich in SFA and omega-3 PUFA exacerbated NSAID-induced small intestinal damage via increased leukocyte infiltration.Importantly,a diet rich in omega-6-PUFA did not aggravate inflammation as monocyte migration was blocked.