Dynamic analysis of BMP‐responsive smad activity in live zebrafish embryos

Bone morphogenetic proteins (BMPs) are critical players in development and disease, regulating such diverse processes as dorsoventral patterning, palate formation, and ossification. These ligands are classically considered to signal via BMP receptor‐specific Smad proteins 1, 5, and 8. To determine t...

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Bibliographic Details
Published in:Developmental dynamics 2011-03, Vol.240 (3), p.682-694
Main Authors: Laux, Derek W., Febbo, Jennifer A., Roman, Beth L.
Format: Article
Language:English
Subjects:
Animals
Bone Morphogenetic Protein Receptors, Type I - genetics
Bone Morphogenetic Protein Receptors, Type I - metabolism
bone morphogenetic proteins
Bone Morphogenetic Proteins - genetics
Bone Morphogenetic Proteins - metabolism
Embryo, Nonmammalian - drug effects
Embryo, Nonmammalian - metabolism
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Immunohistochemistry
In Situ Hybridization
Smad Proteins - genetics
Smad Proteins - metabolism
Smad1 Protein - genetics
Smad1 Protein - metabolism
Smad5 Protein - genetics
Smad5 Protein - metabolism
Smad8 Protein - genetics
Smad8 Protein - metabolism
Smads
transgenic reporter
zebrafish
Zebrafish - embryology
Zebrafish - genetics
Zebrafish - metabolism
Zebrafish Proteins - genetics
Zebrafish Proteins - metabolism
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Summary:Bone morphogenetic proteins (BMPs) are critical players in development and disease, regulating such diverse processes as dorsoventral patterning, palate formation, and ossification. These ligands are classically considered to signal via BMP receptor‐specific Smad proteins 1, 5, and 8. To determine the spatiotemporal pattern of Smad1/5/8 activity and thus canonical BMP signaling in the developing zebrafish embryo, we generated a transgenic line expressing EGFP under the control of a BMP‐responsive element. EGFP is expressed in many established BMP signaling domains and is responsive to alterations in BMP type I receptor activity and smad1 and smad5 expression. This transgenic Smad1/5/8 reporter line will be useful for determining ligand and receptor requirements for specific domains of BMP activity, as well as for genetic and pharmacological screens aimed at identifying enhancers or suppressors of canonical BMP signaling. Developmental Dynamics 240:682–694, 2011. © 2011 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.22558