Proteomes of Host Cell Membranes Modified by Intracellular Activities of Salmonella enterica

Intracellular pathogens need to establish a growth-stimulating host niche for survival and replication. A unique feature of the gastrointestinal pathogen Salmonella enterica serovar Typhimurium is the creation of extensive membrane networks within its host. An understanding of the origin and functio...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2015-01, Vol.14 (1), p.81-92
Main Authors: Vorwerk, Stephanie, Krieger, Viktoria, Deiwick, Jörg, Hensel, Michael, Hansmeier, Nicole
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Cell Line
Endoplasmic Reticulum - metabolism
HeLa Cells
Host-Pathogen Interactions
Humans
Membrane Proteins - metabolism
Mice
Proteome
Salmonella enterica
Salmonella enterica - physiology
Salmonella Infections - metabolism
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Summary:Intracellular pathogens need to establish a growth-stimulating host niche for survival and replication. A unique feature of the gastrointestinal pathogen Salmonella enterica serovar Typhimurium is the creation of extensive membrane networks within its host. An understanding of the origin and function of these membranes is crucial for the development of new treatment strategies. However, the characterization of this compartment is very challenging, and only fragmentary knowledge of its composition and biogenesis exists. Here, we describe a new proteome-based approach to enrich and characterize Salmonella-modified membranes. Using a Salmonella mutant strain that does not form this unique membrane network as a reference, we identified a high-confidence set of host proteins associated with Salmonella-modified membranes. This comprehensive analysis allowed us to reconstruct the interactions of Salmonella with host membranes. For example, we noted that Salmonella redirects endoplasmic reticulum (ER) membrane trafficking to its intracellular niche, a finding that has not been described for Salmonella previously. Our system-wide approach therefore has the potential to rapidly close gaps in our knowledge of the infection process of intracellular pathogens and demonstrates a hitherto unrecognized complexity in the formation of Salmonella host niches.
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M114.041145