The inhibitory effect of colloidal bismuth hydroxide gel on Escherichia coli O157:H7 and on the activity of Shiga toxins

Shiga toxin-producing Escherichia coli (STEC) is the causative agent of hemolytic uremic syndrome (HUS). Colloidal bismuth hydroxide gel (CBHG) is an anti-diarrheal and antisecretory compound, which does not inhibit gastrointestinal motility and reaches an in vivo gut concentration of 10.8 mg/ml of...

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Bibliographic Details
Published in:BMC research notes 2014-12, Vol.7 (1), p.875-875
Main Authors: Subils, Tomás, Casabonne, Cecilia, Balagué, Claudia
Format: Article
Language:English
Subjects:
Antibiotics
Bacterial Adhesion - drug effects
Bacteriology
Bismuth - pharmacology
Colloids
Cytotoxicity
E coli
Escherichia coli
Escherichia coli O157 - drug effects
Escherichia coli O157 - genetics
Gels
Genes, Bacterial
Hydroxides - pharmacology
Immunoenzyme Techniques
Infections
Kidneys
Microbial Sensitivity Tests
Proteins
Shiga Toxins - antagonists & inhibitors
Studies
Toxins
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Summary:Shiga toxin-producing Escherichia coli (STEC) is the causative agent of hemolytic uremic syndrome (HUS). Colloidal bismuth hydroxide gel (CBHG) is an anti-diarrheal and antisecretory compound, which does not inhibit gastrointestinal motility and reaches an in vivo gut concentration of 10.8 mg/ml of bismuth. Its action on bacteria has not been studied. We analyzed its inhibitory effects on STEC, as well as the deactivation of the Shiga toxin (Stx) and its ability to block the spread of genes encoding Stx. We determined a minimum inhibitory concentration and bactericidal concentration for the STEC O157:H7 strain (EDL933), with CBHG and Chobet® bismuth cream with pectin (CBCHP). We analyzed its effect on Stx by means of cytotoxicity assay and ELISA, as well as its effect on the free 933 W Stx phage. Effect on the EDL933 strain: CBHG: MIC 10 mg/ml of bismuth. MIC 6 mg/ml and MBC 15 mg/ml of bismuth. Effect on EDL933 virulence factors: significant decrease in active Stx and 933 W Stx phage titer. ELISA did not find significant differences with treatment. The results obtained may be useful in the development of new therapeutic strategies based on the use of CBHG to prevent or improve the prognosis of HUS, as it can be used to control STEC infections.
ISSN:1756-0500
1756-0500
DOI:10.1186/1756-0500-7-875