Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus

We show that a previously annotated hypothetical protein is the transposase of a new and active IS element, ISCahy1, widespread in Caldicellulosiruptor species. Transposition generated an 11-bp direct repeat at the insertion site in Caldicellulosiruptor hydrothermalis, suggesting a cut-and-paste mec...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2013-05, Vol.40 (5), p.517-521
Main Authors: Chung, Daehwan, Farkas, Joel, Westpheling, Janet
Format: Article
Language:English
Subjects:
Amino acids
Bacteria
Base Sequence
Biochemistry
Bioinformatics
Biological and medical sciences
Biomass energy
Biomedical and Life Sciences
Biotechnology
Caldicellulosiruptor
classification
DNA Transposable Elements
DNA Transposable Elements - genetics
Fundamental and applied biological sciences. Psychology
Genetic Engineering
genetics
Genome, Bacterial
Genome, Bacterial - genetics
Genomes
Gram-Positive Bacteria
Gram-Positive Bacteria - classification
Gram-Positive Bacteria - genetics
Inorganic Chemistry
Life Sciences
metabolism
Microbiology
Molecular Sequence Data
Natural products
nucleotide sequences
Proteins
Short Communication
Studies
surveys
Transposases
Transposases - genetics
Transposases - metabolism
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Summary:We show that a previously annotated hypothetical protein is the transposase of a new and active IS element, ISCahy1, widespread in Caldicellulosiruptor species. Transposition generated an 11-bp direct repeat at the insertion site in Caldicellulosiruptor hydrothermalis, suggesting a cut-and-paste mechanism. The discovery of an active insertion sequence in Caldicellulosiruptor species led to a survey of potential IS elements in the genome sequences of eight Caldicellulosiruptor species that identified several new elements, including one novel to this genus.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-013-1244-z