Stability of 3-bromotyrosine in serum and serum 3-bromotyrosine concentrations in dogs with gastrointestinal diseases

BACKGROUND: 3-Bromotyrosine (3-BrY) is a stable product of eosinophil peroxidase and may serve as a marker of eosinophil activation. A gas chromatography/mass spectrometry method to measure 3-BrY concentrations in serum from dogs has recently been established and analytically validated. The aims of...

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Published in:BMC veterinary research 2015-01, Vol.11 (1), p.5-5
Main Authors: Sattasathuchana, Panpicha, Grützner, Niels, Lopes, Rosana, Guard, Blake C, Suchodolski, Jan S, Steiner, Jörg M
Format: Article
Language:English
Subjects:
Animals
Biomarkers
blood serum
Care and treatment
Case-Control Studies
Chromatography
Diagnosis
Dog Diseases - blood
Dogs
enteritis
eosinophils
Eosinophils - metabolism
Female
gas chromatography
gastroenteritis
Gastrointestinal Diseases - blood
Gastrointestinal Diseases - metabolism
Gastrointestinal Diseases - veterinary
gastrointestinal system
Male
mass spectrometry
Medical research
Medicine, Experimental
Pancreatitis
patients
peroxidase
Risk factors
Tyrosine - analogs & derivatives
Tyrosine - blood
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Summary:BACKGROUND: 3-Bromotyrosine (3-BrY) is a stable product of eosinophil peroxidase and may serve as a marker of eosinophil activation. A gas chromatography/mass spectrometry method to measure 3-BrY concentrations in serum from dogs has recently been established and analytically validated. The aims of this study were to determine the stability of 3-BrY in serum, to determine the association between peripheral eosinophil counts and the presence of an eosinophilic infiltrate in the gastrointestinal tract, and to compare serum 3-BrY concentrations in healthy dogs (n = 52) and dogs with eosinophilic gastroenteritis (EGE; n = 27), lymphocytic-plasmacytic enteritis (LPE; n = 25), exocrine pancreatic insufficiency (EPI; n = 26), or pancreatitis (n = 27). RESULTS: Serum 3-BrY concentrations were stable for up to 8, 30, and 180 days at 4°C, −20°C, and −80°C, respectively. There was no significant association between peripheral eosinophil count and the presence of eosinophils in the GI tissues (P = 0.1733). Serum 3-BrY concentrations were significantly higher in dogs with EGE (median [range] = 5.04 [≤0.63-26.26] μmol/L), LPE (median [range] = 3.60 [≤0.63-15.67] μmol/L), and pancreatitis (median [range] = 1.49 [≤0.63-4.46] μmol/L) than in healthy control dogs (median [range] = ≤0.63 [≤0.63-1.79] μmol/L; P 
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-015-0321-0