A validation study: assessing the reliability of the hand held StatStripXPress lactate meter to test lactate in amniotic fluid

The level of lactate in amniotic fluid may provide useful clinical information when assessing whether a woman in labour is experiencing labour dystocia. If so, a rapid, reliable method to assess the concentration of amniotic fluid lactate at the bedside will be required in order to be clinically rel...

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Bibliographic Details
Published in:BMC research notes 2014-12, Vol.7 (1), p.935-935
Main Authors: Hall, Beverley, Wong, Diana D, Rawlinson, William D, Tracy, Mark B, Tracy, Sally K
Format: Article
Language:English
Subjects:
Adult
Amniocentesis
Amniotic Fluid - chemistry
Computers, Handheld - standards
Dystocia - diagnosis
Dystocia - metabolism
Dystocia - physiopathology
Ethics
Female
Hospitals
Humans
Labor, Obstetric - metabolism
Laboratories
Lactic Acid - analysis
Lactic Acid - metabolism
Linear Models
Medical research
Point-of-Care Systems - standards
Pregnancy
Sensitivity and Specificity
Short Report
Womens health
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Summary:The level of lactate in amniotic fluid may provide useful clinical information when assessing whether a woman in labour is experiencing labour dystocia. If so, a rapid, reliable method to assess the concentration of amniotic fluid lactate at the bedside will be required in order to be clinically relevant. To assess efficacy, we compared the hand held StatStripXPreass lactate meter (Nova Biomedical) to the reference laboratory analyser ABX Pentra 400 (Horiba) in a controlled environment. Baseline biological lactate concentration was measured in triplicate and samples of a known quantity of thawed amniotic fluid spiked with lactate substrate (62 mmol/L) from the LDH12 kit (Roche, SUI) to yield a predetermined lactate concentration above baseline then measured in triplicate. Deming Regression was used to determine the linear agreement and a Bland Altman plot used to determine the paired agreement across the range of values. The mean difference with Bland-Altman plot between hand held meter and lab instrument was -1.0 mmol/L (SD 3.0 mmol/L) with 95% CI limits of agreement between -6.9 mmol/L to 4.9 mmol/L. The Deming regression co-efficient or slope of agreement was 0.91 (SD of 0.21). The measurement of amniotic fluid lactate using the StatStripXPress hand held meter was reliable compared to reference laboratory methods for measuring lactate levels in amniotic fluid.
ISSN:1756-0500
1756-0500
DOI:10.1186/1756-0500-7-935