Screening of gene-associated polymorphisms by use of in-gel competitive reassociation and EST (cDNA) array hybridization

In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymor...

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Bibliographic Details
Published in:Genome research 2003-03, Vol.13 (3), p.492-495
Main Authors: Gotoh, Koshichi, Oishi, Michio
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Binding, Competitive - genetics
DNA, Complementary - genetics
DNA, Plant - genetics
Electrophoresis, Agar Gel - methods
Expressed Sequence Tags
Genes, Plant - genetics
Genetic Markers - genetics
Genetic Testing - methods
Methods
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis - methods
Polymorphism, Genetic - genetics
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Summary:In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.
ISSN:1088-9051
1054-9803
1549-5469
DOI:10.1101/gr.434103