Phylogenetic Relationship of Phosphate Solubilizing Bacteria according to 16S rRNA Genes

Phosphate solubilizing bacteria (PSB) can convert insoluble form of phosphorous to an available form. Applications of PSB as inoculants increase the phosphorus uptake by plant in the field. In this study, isolation and precise identification of PSB were carried out in Malaysian (Serdang) oil palm fi...

Full description

Saved in:
Bibliographic Details
Published in:BioMed research international 2015-01, Vol.2015 (2015), p.1-5
Main Authors: Javadi Nobandegani, Mohammad Bagher, Yun, Wong Mui, Saud, Halimi Mohd
Format: Article
Language:English
Subjects:
Alcaligenes faecalis
Bacillus cereus
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Biomedical research
Chemical elements
Cluster Analysis
Deoxyribonucleic acid
DNA
Firmicutes
Genes
Genes, Bacterial
Genetic aspects
Genetic diversity
Health aspects
Host-bacteria relationships
Methods
Microbial colonies
Microorganisms
Observations
Phosphates - metabolism
Phosphorus
Phylogenetics
Phylogeny
Polymerase Chain Reaction
Proteobacteria
RNA sequencing
RNA, Ribosomal, 16S - genetics
Serratia plymuthica
Soil Microbiology
Solubility
Vagococcus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Phosphate solubilizing bacteria (PSB) can convert insoluble form of phosphorous to an available form. Applications of PSB as inoculants increase the phosphorus uptake by plant in the field. In this study, isolation and precise identification of PSB were carried out in Malaysian (Serdang) oil palm field (University Putra Malaysia). Identification and phylogenetic analysis of 8 better isolates were carried out by 16S rRNA gene sequencing in which as a result five isolates belong to the Beta subdivision of Proteobacteria, one isolate was related to the Gama subdivision of Proteobacteria, and two isolates were related to the Firmicutes. Bacterial isolates of 6upmr, 2upmr, 19upmnr, 10upmr, and 24upmr were identified as Alcaligenes faecalis. Also, bacterial isolates of 20upmnr and 17upmnr were identified as Bacillus cereus and Vagococcus carniphilus, respectively, and bacterial isolates of 31upmr were identified as Serratia plymuthica. Molecular identification and characterization of oil palm strains as the specific phosphate solubilizer can reduce the time and cost of producing effective inoculate (biofertilizer) in an oil palm field.
ISSN:2314-6133
2314-6141
DOI:10.1155/2015/201379