Significant N2 fixation by heterotrophs, photoheterotrophs and heterocystous cyanobacteria in two temperate estuaries

• Published in: The ISME Journal 2015-02, Vol.9 (2), p.273-285
• Main Authors: Bentzon-Tilia, Mikkel, Traving, Sachia J, Mantikci, Mustafa, Knudsen-Leerbeck, Helle, Hansen, Jørgen LS, Markager, Stiig, Riemann, Lasse
• Format: Article
• Language: English
• Subjects: 13/31; 45/22; 45/23; 45/77; 45/90; 631/326/2565/855; Aquatic environment; Biomedical and Life Sciences; Cyanobacteria - genetics; Cyanobacteria - metabolism; Ecology; Estuaries; Evolutionary Biology; Heterotrophic Processes; Life Sciences; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Nitrogen fixation; Nitrogen Fixation - genetics; Original; original-article; Oxidoreductases - genetics; Phototrophic Processes; Plankton - genetics; Plankton - metabolism
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/ismej.2014.119

Nitrogen (N) fixation is fueling planktonic production in a multitude of aquatic environments. In meso- and poly-haline estuaries, however, the contribution of N by pelagic N 2 fixation is believed to be insignificant due to the high input of N from land and the presumed absence of active N 2 -fixing organisms. Here we report N 2 fixation rates, nifH gene composition and nifH gene transcript abundance for key diazotrophic groups over 1 year in two contrasting, temperate, estuarine systems: Roskilde Fjord (RF) and the Great Belt (GB) strait. Annual pelagic N 2 fixation rates averaged 17 and 61 mmol N m −2 per year at the two sites, respectively. In RF, N 2 fixation was mainly accompanied by transcripts related to heterotrophic (for example, Pseudomonas sp.) and photoheterotrophic bacteria (for example, unicellular diazotrophic cyanobacteria group A). In the GB, the first of two N 2 fixation peaks coincided with a similar nifH -expressing community as in RF, whereas the second peak was synchronous with increased nifH expression by an array of diazotrophs, including heterotrophic organisms as well as the heterocystous cyanobacterium Anabaena . Thus, we show for the first time that significant planktonic N 2 fixation takes place in mesohaline, temperate estuaries and that the importance of heterotrophic, photoheterotrophic and photosynthetic diazotrophs is clearly variable in space and time.