Enhancement of leukocyte adhesion after percutaneous irradiation in rats with hepatocellular carcinoma

• Published in: World journal of gastroenterology : WJG 2005-04, Vol.11 (13), p.1991-1994
• Main Authors: Maksan, Sasa-Marcel, Schmidt, Eduard, Ryschich, Eduard, Harms, Wolfgang, Schmidt, Jan
• Format: Article
• Language: English
• Subjects: Animals; Brief Reports; Carcinoma, Hepatocellular - immunology; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - radiotherapy; Cell Adhesion - immunology; Cell Adhesion - radiation effects; Cell Movement - immunology; Cell Movement - radiation effects; Disease Models, Animal; Leukocytes - cytology; Leukocytes - radiation effects; Liver Neoplasms, Experimental - immunology; Liver Neoplasms, Experimental - pathology; Liver Neoplasms, Experimental - radiotherapy; Male; Radiotherapy - methods; Rats; Rats, Inbred ACI; 动物实验; 放射治疗; 白细胞; 肝细胞癌; 营养支持
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v11.i13.1991

AIM: To evaluate the effects of percutaneous radiation on leukocyte-endothelium interaction (LEI) in experimental hepatocellular carcinoma (HCC). METHODS: Twelve ACI rats underwent HCC-inoculation, six of which on day 12 received low-dose external radiation and six did not. After 12 h intravital microscopy was performed. RESULTS: LEI was significantly reduced in tumor tissue. However, irradiation of liver sinusoids and tumor tissue with 6 Gy led to a significant activation of leukocyte adhesion in the tumor with a marked increase of the proinflammatory cytokine TNF-α. CONCLUSION: The findings indicate that the immunological tumor-endothelial barrier can be overcome by external irradiation.