Suppression of gastric cancer growth by adenovirus-mediated transfer of the PTEN gene

AIM: To investigate the tumor-suppressive effect of the phosphatase and tensin homologue deleted from chromosome (PTEN) in human gastric cancer cells that were wild type for PTEN.METHODS: Adenoviruses expressing PTEN or luciferase as a control were introduced into gastric cancer cells. The effect of...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2005-04, Vol.11 (15), p.2224-2229
Main Authors: Hang, Ying, Zheng, Yong-Chen, Cao, Yan, Li, Qing-Shan, Sui, Yu-Jie
Format: Article
Language:English
Subjects:
Adenocarcinoma, Mucinous - genetics
Adenocarcinoma, Mucinous - secondary
Adenocarcinoma, Mucinous - therapy
Adenoviridae - genetics
Animals
Cell Division
Cell Line, Tumor
Gastric Cancer
Gene Transfer Techniques
Genetic Therapy - methods
Humans
Lymphatic Metastasis
Mice
Mice, Nude
Phosphoric Monoester Hydrolases - genetics
PTEN
PTEN Phosphohydrolase
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach Neoplasms - therapy
Tumor Suppressor Proteins - genetics
Xenograft Model Antitumor Assays
肿瘤生长
肿瘤转移
胃肿瘤
腺病毒
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Summary:AIM: To investigate the tumor-suppressive effect of the phosphatase and tensin homologue deleted from chromosome (PTEN) in human gastric cancer cells that were wild type for PTEN.METHODS: Adenoviruses expressing PTEN or luciferase as a control were introduced into gastric cancer cells. The effect of exogenous PTEN gene on the growth and apoptosis of gastric cancer cells that are wtPTEN were examined in vitro and in vivo.RESULTS: Adenovirus-mediated transfer of PTEN (Ad-PTEN) suppressed cell growth and induced apoptosis significantly in gastric cancer cells (MGC-803, SGC-7901) carrying wtPTEN in comparison with that in normal gastric epithelial cells (GES-1) carrying wtPTEN. This suppression was induced through downregulation of the Akt/PKB pathway, dephosphorylation of focal adhesion kinase and mitogen-activated protein kinase and cell-cycle arrest at the G2/M phase but not at the G1 phase. Furthermore, treatment of human gastric tumor xenografts (MGC-803, SGC-7901) with Ad-PTEN resulted in a significant (P
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v11.i15.2224