Selective scattering spectra as an approach to internal structure of granal and agranal chloroplasts

Selective scattering spectra of granal and agranal chloroplasts were measured in the red spectral region and compared with calculations based on the Mie theory. The spectra were influenced considerably by the intactness and ultrastructural pattern of the chloroplasts. It was demonstrated that the sp...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1977-04, Vol.74 (4), p.1455-1457
Main Authors: Bialek, G.E, Horvath, G, Garab, Gy.I, Mustardy, L.A, Faludi-Daniel, A
Format: Article
Language:English
Subjects:
Absorption spectra
Bundle sheath cells
Chlorophyll - analysis
Chlorophylls
Chloroplasts
Chloroplasts - ultrastructure
corn
Cytoplasmic Granules - ultrastructure
Grana
Light refraction
Mathematics
Mesophyll
Plants
Protoplasts
Scattering, Radiation
Spectrophotometry
Suspended solids
Wavelengths
Zea mays
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Description
Summary:Selective scattering spectra of granal and agranal chloroplasts were measured in the red spectral region and compared with calculations based on the Mie theory. The spectra were influenced considerably by the intactness and ultrastructural pattern of the chloroplasts. It was demonstrated that the spectra consist of two components: one attributable to grana and the other, to single lamellae. The dependence of the selective scattering spectra on the ultrastructural characteristics offers a convenient method for monitoring the quality of chloroplast preparations by a procedure much faster than electron microscopy.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.74.4.1455